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Gold nanoprobe functionalized with specific fusion protein selection from phage display and its application in rapid, selective and sensitive colorimetric biosensing of Staphylococcus aureus

2016; Elsevier BV; Volume: 82; Linguagem: Inglês

10.1016/j.bios.2016.03.075

1873-4235

Pei Liu, Lei Han, Fei Wang, Valery A. Petrenko, Aihua Liu,

SARS-CoV-2 detection and testing

Staphylococcus aureus (S. aureus) is one of the most ubiquitous pathogens in public healthcare worldwide. It holds great insterest in establishing robust analytical method for S. aureus. Herein, we report a S. aureus-specific recognition element, isolated from phage monoclone GQTTLTTS, which was selected from f8/8 landscape phage library against S. aureus in a high-throughput way. By functionalizing cysteamine (CS)-stabilized gold nanoparticles (CS-AuNPs) with S. aureus-specific pVIII fusion protein (fusion-pVIII), a bifunctional nanoprobe ([email protected]) for S. aureus was developed. In this strategy, the [email protected] could be induced to aggregate quickly in the presence of target S. aureus, resulting in a rapid colorimetric response of gold nanoparticles. More importantly, the as-designed probe exhibited excellent selectivity over other bacteria. Thus, the [email protected] could be used as the indicator of target S. aureus. This assay can detect as low as 19 CFU mL−1 S. aureus within 30 min. Further, this approach can be applicable to detect S. aureus in real water samples. Due to its sensitivity, specificity and rapidness, this proposed method is promising for on-site testing of S. aureus without using any costly instruments.