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Uncaria tomentosa Willd. DC (Cat�s claw) immunostimulatory activity in splenic B lymphocytes

2013; Frontiers Media; Volume: 4; Linguagem: Inglês

10.3389/conf.fimmu.2013.02.01199

1664-3224

Zegarra Ruiz Daniel, Aguilar Olano Jose,

Berberine and alkaloids research

Event Abstract Back to Event Uncaria tomentosa Willd. DC (Cat’s claw) immunostimulatory activity in splenic B lymphocytes Daniel F. Zegarra Ruiz1 and Jose L. Aguilar Olano1* 1 Universidad Peruana Cayetano Heredia, Department of Cellular and Molecular Sciences, Peru INTRODUCTION: B lymphocytes play a crucial role in humoral immunity; their activation provides the basis of immunizations against different diseases. This study assessed the immunostimulatory activity of a Cat’s claw (Uncaria tomentosa Willd. DC) hydro-alcoholic extract with 5% of pentacyclic oxindole alkaloids (UT) on splenic B cells. OBJECTIVES: 1. Calculate the UT IC50 value in splenocytes. 2. Evaluate the effect of UT in mononuclear cells activation. 3. Evaluate the effect in splenic B cells. METHODOLOGY: Spleens were obtained from 7-week-old C57BL/6 mice. Splenocytes were treated with different concentrations of UT. IC50 value was calculated after 72h UT incubation and adding 7AAD. Subsequently, splenocytes were stimulated under the same conditions and the activation of mononuclear cells and B lymphocytes (B220+/CD25CD69+/+) was assessed by flow cytometry. Data was analyzed with Graphpad Prism 5. RESULTS: The IC50 value obtained for UT in splenocytes was 140.9ug/ml. A dose-dependent activation was shown after UT stimulation. Mononuclear cells were significantly activated by UT (18.3% for UT 90ug/ml vs. 2.5% CN). Furthermore, B lymphocytes presented a similar behavior (20.45% for UT 90ug/ml vs. 1.8% CN); however, their proliferation levels remained the same as shown in the population percentage for all the treated and control groups (65% of total splenocytes). CONCLUSION: These results support the immunostimulatory activity of UT and demonstrate specificity towards B cell activation, suggesting that UT could be used as a potent adjuvant to trigger humoral immunity. Acknowledgements This research project could have not been developed without the supervision and assistance provided by Vrajesh Parekh, PhD. and Prof. Luc Van Kaer, PhD. References 1. Piscoya J, Rodríguez Z, Bustamante SA, Okuhama NN, Miller MJS, Sandoval M. Efficacy and safety of freeze-dried cat´s claw in osteoarthritis of the knee. Mechanisms of action of species of Uncaria guianensis. Inflamm Res 2001;50:442-8 2. Castañeda O, León G, León D, et al. Uña de Gato en Artritis Reumatoide: Estudio doble ciego, en comparación con placebo. Rev Peru Reumatol 1998;4:15-21 3. Mur E, Hartig F, Eibl G, Schirmer M.. Randomized Double Blind Trial of an Extract from the Pentacyclic Alkaloid-Chemotype of Uncaria tomentosa for the Treatment of Rheumatoid Arthritis. J Rheumatol 2002;29:656-8 4. Aguilar JL, Rojas P, Marcelo A, Plaza A, Bauer R, Reininger E, Klaas CA, Merfort I. Anti-inflammatory activity of two different extracts of Uncaria tomentosa (Rubiaceae). J Ethnopharmacol 2002; 81:271-6 5. Rojas PA. Producción del factor de necrosis tumoral alfa en un modelo in-vivo y su inhibición por un extracto de Uncaria tomentosa (Uña de gato). Tesis de Licenciatura. UPCH. Lima, Perú. 2002 6. Lozada-Requena I. Efecto inmunomodulador de capsaicina y Uncaria tomentosa (uña de gato) sobre la producción del factor de necrosis tumoral de tipo alfa e interleuquina 10 y sobre la inducción de apoptosis en células mononucleares de sangre periférica humanas [Tesis de Magister]. Lima-Perú: Biblioteca Central, Universidad Peruana Cayetano Heredia; 2006 7. Núñez C, Lozada-Requena IA, Akamine I, Aguilar JL. Efecto de Uncaria tomentosa (Uña de gato) sobre la población y activación de células dendríticas en pacientes con artritis reumatoidea [Artículo enviado a publicación] 2008. 8. Quevedo H, Vidal L, Castañeda L. Artritis reumatoide: una enfermedad con prevalencia homogénea. Rev. Perú Reum. 1995; 1(1): 40 – 1. Keywords: Uncaria tomentosa, spleen mononuclear leukocytes, B lymphocytes, Cell activation, IC50 Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Adaptive Immunity Citation: Zegarra Ruiz DF and Aguilar Olano JL (2013). Uncaria tomentosa Willd. DC (Cat’s claw) immunostimulatory activity in splenic B lymphocytes. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01199 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 18 Jul 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Jose L Aguilar Olano, Universidad Peruana Cayetano Heredia, Department of Cellular and Molecular Sciences, Lima, Peru, jose.aguilar@upch.pe Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. 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