Efficient identification and analysis of chemical exchange in biomolecules by R 1ρ relaxation dispersion with Amaterasu
2016; Oxford University Press; Volume: 32; Issue: 16 Linguagem: Inglês
10.1093/bioinformatics/btw188
ISSN1367-4811
AutoresErik Walinda, Daichi Morimoto, Mayu Nishizawa, Masahiro Shirakawa, Kenji Sugase,
Tópico(s)NMR spectroscopy and applications
ResumoWe introduce here a novel acquisition and processing methodology for cross-polarization based 1D rotating-frame relaxation dispersion NMR experiments. This easy-to-use protocol greatly facilitates the screening, acquisition, processing and model fitting of large on- and off-resonance R1ρ relaxation dispersion NMR datasets in an automated manner for the analysis of chemical exchange phenomena in biomolecules.The Amaterasu package including the spreadsheet, Bruker pulse programs and analysis software is available at www.moleng.kyoto-u.ac.jp/∼moleng_01/amaterasu: sugase@moleng.kyoto-u.ac.jp.
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