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Vancomycin Microbial Assay Using Kinetic-Reading Microplate System

2013; Bentham Science Publishers; Volume: 9; Issue: 2 Linguagem: Inglês

10.2174/1573412911309020007

1875-676X

Túlia de Souza Botelho, Felipe Rebello Lourenço, Terezinha de Jesus Andreoli Pinto,

Probiotics and Fermented Foods

The aim of this work was to develop a microbiological assay of vancomycin employing microplates and kinetic reading mode, and to validate the developed method by assessing of specificity and selectivity, linearity, linear range, accuracy and precision parameters. Established conditions comprised a vancomycin standard-curve in concentrations from 0.6 to 1.0 μg mL-1, and soybean-casein broth inoculated with 5% of Bacillus subtilis (ATCC 6633) suspension. Satisfactory results were obtained after a 5 hour incubation. The developed method showed appropriate selectivity and linearity in the range from 0.6 to 1.0 μg mL-1, respectively, satisfactory accuracy (recovery = 97.0%) and precision (RSD = 7.8%). Keywords: Agar diffusion, Antibiotic assay, Glycopeptide antibiotic, Kinetic reading, Method development, Method validation, Microbial assay, Microplate assay, Turbidimetric assay, Vancomycin