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A Modified Plastic Petri Dish for Cell and Tissue Cultures.

1961; SAGE Publishing; Volume: 106; Issue: 4 Linguagem: Inglês

10.3181/00379727-106-26480

1535-3702

William Cooper,

3D Printing in Biomedical Research

For many years the 60 mm glass Petri dish has served as a standard culture vessel for maintenance of a variety of cell strains and tissue explants in vitro. Monolayer cultures have been maintained on the inside bottom of the Petri dish overlaid by a prescribed volume of liquid medium whose pH is maintained by the humidified 5% CO2 environment of the incubator in which the culture dishes are placed. Observations of the growing cultures require an inverted microscope or replacement of the dish top with a device enabling one to bring the objective close enough so that the cells can be viewed with an ordinary microscope. Both sytems leave much to be desired regarding the cytological resolution obtained. The culture dish herein described has many features which tend to overcome some of the problems encountered in growing monolayer cultures in conventional dishes.The culture dish is made of a pre-sterilized polystyrene plastic. The lid has a sharply recessed area 40 mm in diameter (Fig. 1. B). The sides of the bottom (Fig. 1, C) of the dish are tapered to facilitate their stacking or filing for maintaining permanent records of experiments. The optical working distance from top of the lid to inside bottom of the dish (cell surface) is 2.6 mm. This enables one to study individual cells of the monolayer by using an ordinary light microscope or phase microscope (Fig. 2). When making observations with an inverted microscope (Fig. 3), the recessed lid permits the phase condenser to be focused on the cell surface, thereby producing much better definition of cell structure at higher magnifications. All of these optical procedures do not increase the chance of contamination since all are done with the recessed lid in place.