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New Fluorescent and Colorimetric Probe for Cyanide: Direct Reactivity, High Selectivity, and Bioimaging Application

2012; American Chemical Society; Volume: 4; Issue: 8 Linguagem: Inglês

10.1021/am3010412

1944-8252

Xiaohong Cheng, Runli Tang, Huizhen Jia, Jun Feng, Jingui Qin, Zhen Li,

Sulfur Compounds in Biology

Taking advantage of the special nucleophilicity of cyanide, a new ratiometric fluorescent and colorimetric probe (Coum-1) was designed by tuning the π–conjugated bridge to affect the intramolecular charge transfer efficiency. Upon the addition of CN– anion, the probe displayed very large blue-shift in both fluorescence (90 nm) and absorption (90 nm) spectra, with the detection limit of 800 nM. Other anions gave nearly no interference. Furthermore, Coum-1 was successfully applied to the fluorescent microscopic imaging for the detection of CN– in HeLa cells.