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Preliminary evaluation of antigens from Paracoccidioides brasiliensis responsible for protective immune response induced in P. brasiliensis-infected mice by treatment with complete Freund�s adjuvant.

2013; Frontiers Media; Volume: 4; Linguagem: Inglês

10.3389/conf.fimmu.2013.02.00460

1664-3224

Landgraf Taise, Fernandes Fabr�cio, Gabriela Peron, Coelho-Castelo Arlete, Panunto-Castelo Ademilson,

T-cell and Retrovirus Studies

Event Abstract Back to Event Preliminary evaluation of antigens from Paracoccidioides brasiliensis responsible for protective immune response induced in P. brasiliensis-infected mice by treatment with complete Freund’s adjuvant. Taise N. Landgraf1*, Fabrício F. Fernandes1, Gabriela Peron1, Arlete A. Coelho-Castelo1 and Ademilson Panunto-Castelo2 1 University of São Paulo, Biochemistry and Immunology, Brazil 2 University of São Paulo, Biology, Brazil Paracoccidioidomycosis (PCM) is an infectious disease caused by the dimorphic fungus Paracoccidioides brasiliensis. Among the deep mycoses, PCM is the most prevalent in Latin America and represents a major public health problem in countries where there is a higher incidence of the disease. In Brazil, PCM corresponds to the eighth leading cause of death among chronic or recurrent infections and parasitic diseases. Previous results from our group have shown that complete Freund’s adjuvant (CFA) has a therapeutic effect in P. brasiliensis-infected mice. In the current study, we propose to isolate P. brasiliensis antigens associated with the protective immune response induced by CFA in infected mice. Exoantigens (ExoAg) and somatic antigens (SoAg) were isolated from yeast cells of virulent Pb18 strain of P. brasiliensis, and electrophoresis of the preparations showed high complexity of proteins. Both antigenic preparations were able to induce delayed-type hypersensitive (DTH) reactions in BALB/c mice previously infected with the fungus, though more prominent and significant DTH reactions were elicited when the mice were treated with CFA. Moreover, these preparations induced a proliferation in CFSE-labeled CD3+ spleen cells from mice infected with P. brasiliensis and treated with CFA significantly higher than those from only infected mice. These results suggest that ExoAg and SoAg contain antigens that are potential target to immunotherapeutic intervention in PCM. Others assays in vitro and in vivo are being conducted to isolate antigens from P. brasiliensis capable of inducing and/or enhancing the development of a protective cellular immune response. References Autran, B., G. Carcelain, et al. Therapeutic vaccines for chronic infections. Science, v.305, n.5681, p.205-8. 2004. Bedoya, V., J. G. Mcewen, et al. Pathogenesis of paracoccidioidomycosis: a histopathological study of the experimental murine infection. Mycopathologia, v.94, n.3, p.133-44. 1986. Bisio, L. C., S. P. Silva, et al. A new Paracoccidioides brasiliensis 70-kDa heat shock protein reacts with sera from paracoccidioidomycosis patients. Med Mycol, v.43, n.6, p.495-503. 2005. Borges-Walmsley, M. I., D. Chen, et al. The pathobiology of Paracoccidioides brasiliensis. Trends Microbiol, v.10, n.2, p.80-7. 2002. Camargo, Z. P., C. Unterkircher, et al. Identification of antigenic polypeptides of Paracoccidioides brasiliensis by immunoblotting. J Med Vet Mycol, v.27, n.6, p.407-12. 1989. Cheng, C., P. Jain, et al. A TLR2 agonist is a more effective adjuvant for a Chlamydia major outer membrane protein vaccine than ligands to other TLR and NOD receptors. Vaccine, v.29, n.38, p.6641-9, 2011. Coutinho, Z. F., D. Silva, et al. Paracoccidioidomycosis mortality in Brazil (1980-1995). Cad Saude Publica, v.18, n.5, p.1441-54. 2002. Cox, J. C. e A. R. Coulter. Adjuvants--a classification and review of their modes of action. Vaccine, v.15, n.3, p.248-56. 1997. De Oliveira, L. L., K. C. Coltri, et al. T helper 1-inducing adjuvant protects against experimental paracoccidioidomycosis. PLoS Negl Trop Dis, v.2, n.3, p.e183. 2008. Deepe, G. S., Jr. Preventative and therapeutic vaccines for fungal infections: from concept to implementation. Expert Rev Vaccines, v.3, n.6, p.701-9. 2004. Embry, C. A., L. Franchi, et al. Mechanism of impaired NLRP3 inflammasome priming by monophosphoryl lipid A. Sci Signal, v.4, n.171, p.28. 2011. Ferreira, K. S. e S. R. Almeida. Immunization of susceptible mice with gp43-pulsed dendritic cells induce an increase of pulmonary Paracoccidioidomycosis. Immunol Lett, v.103, n.2, p.121-6. 2006. Franco, M., M. T. Peraçoli, et al. Host-parasite relationship in paracoccidioidomycosis. Curr Top Med Mycol, v.5, p.115-49. 1993. Girois, S. B., F. Chapuis, et al. Adverse effects of antifungal therapies in invasive fungal infections: review and meta-analysis. Eur J Clin Microbiol Infect Dis, v.24, n.2, p.119-30. 2005. Hunter, R. L. Overview of vaccine adjuvants: present and future. Vaccine, v.20 Suppl 3, p.S7-12. 2002. Kashino, S. S., R. A. Fazioli, et al. Resistance to Paracoccidioides brasiliensis infection is linked to a preferential Th1 immune response, whereas susceptibility is associated with absence of IFN-gamma production. J Interferon Cytokine Res, v.20, n.1, p.89-97. 2000. Martinez, R. Paracoccidioidomycosis: the dimension of the problem of a neglected disease. Rev Soc Bras Med Trop, v.43, n.4, p.480. 2010. Mccluskie, M. J. e R. D. Weeratna. Novel adjuvant systems. Curr Drug Targets Infect Disord, v.1, n.3, p.263-71. 2001. Negroni, R. Paracoccidioidomycosis (South American blastomycosis, Lutz's mycosis). Int J Dermatol, v.32, n.12, p.847-59. 1993. Panunto-Castelo, A., G. Freitas-Da-Silva, et al. Paracoccidioides brasiliensis exoantigens: recognition by IgG from patients with different clinical forms of paracoccidioidomycosis. Microbes Infect, v.5, n.13, p.1205-11. 2003. Petrovsky, N. e J. C. Aguilar. Vaccine adjuvants: current state and future trends. Immunol Cell Biol, v.82, n.5, p.488-96. 2004. Restrepo, A., G. Benard, et al. Pulmonary paracoccidioidomycosis. Semin Respir Crit Care Med, v.29, n.2, p.182-97. 2008. Stills, H. F., Jr. Adjuvants and antibody production: dispelling the myths associated with Freund's complete and other adjuvants. ILAR J, v.46, n.3, p.280-93. 2005. Keywords: Paracoccidioides brasiliensis, Paracoccidioidomycosis, Fungal antigens, Immunotherapy, adjuvants Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Landgraf TN, Fernandes FF, Peron G, Coelho-Castelo AA and Panunto-Castelo A (2013). Preliminary evaluation of antigens from Paracoccidioides brasiliensis responsible for protective immune response induced in P. brasiliensis-infected mice by treatment with complete Freund’s adjuvant.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00460 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 01 Apr 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Taise N Landgraf, University of São Paulo, Biochemistry and Immunology, RIBEIRAO PRETO, sp, 14049-900, Brazil, taise.landgraf@usp.br Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Taise N Landgraf Fabrício F Fernandes Gabriela Peron Arlete A Coelho-Castelo Ademilson Panunto-Castelo Google Taise N Landgraf Fabrício F Fernandes Gabriela Peron Arlete A Coelho-Castelo Ademilson Panunto-Castelo Google Scholar Taise N Landgraf Fabrício F Fernandes Gabriela Peron Arlete A Coelho-Castelo Ademilson Panunto-Castelo PubMed Taise N Landgraf Fabrício F Fernandes Gabriela Peron Arlete A Coelho-Castelo Ademilson Panunto-Castelo Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.