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In situ Evaluation of Protein Adsorption on Polymer Films Coated over Piezoelectric Quartz Resonators.

1996; Membrane Society of Japan; Volume: 21; Issue: 1 Linguagem: Inglês

10.5360/membrane.21.74

1884-6440

Masao Sudoh, Takako Aonuma, Yoshiko Tsubaki, Kazuyoshi Ohashi, Motohiko Kimura,

Microfluidic and Capillary Electrophoresis Applications

Adsorption of plasma protein on polymer films was measured by using a quartz-crystal microbalance in a flow cell. Piezoelectric AT-cut quartz resonators in contact with aqueous protein solution, coated with polymer films of poly (vinyl alcohol), polyurethane, silicon and Nafion, exhibit frequency changes according to mass changes by adsorption and desorption of the protein on the film. The mass change was determined by the frequency response according to the Sauerbrey relationship. Adsorption quilibria of bovin serum albumin and fibrinogen on polymer filmes were well correlated by using Langmuir isothemal equations. The protein mass adsorbed on the Nafion was largest, followed by silicon, polyurethane and Poly (vinyl alcohol) films. In the exchange adsorption/desorption experiment, the albumin on the Nafion was found to keep adsorbed on the film in aqueous fibrinogen solution. Real-time measurements of adsorption and desorption properties of protions were demonstrated and revealed a useful applicability of a quartz-cryatal microbalance.