Artigo Revisado por pares

Effects of experimentally induced infections of goldfish Carassius auratus with cyprinid herpesvirus 2 (CyHV-2) at various water temperatures

2014; Inter-Research; Volume: 110; Issue: 3 Linguagem: Inglês

10.3354/dao02759

ISSN

1616-1580

Autores

Takafumi Ito, Yukio Maeno,

Tópico(s)

Herpesvirus Infections and Treatments

Resumo

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 110:193-200 (2014) - DOI: https://doi.org/10.3354/dao02759 Effects of experimentally induced infections of goldfish Carassius auratus with cyprinid herpesvirus 2 (CyHV-2) at various water temperatures Takafumi Ito1,*, Yukio Maeno2 1Tamaki Laboratory, Aquatic Animal Health Division, National Research Institute of Aquaculture, Fisheries Research Agency, 224-1 Hiruta, Tamaki, Mie 519-0423, Japan 2Aquatic Animal Health Division, National Research Institute of Aquaculture, Fisheries Research Agency, 422-1 Nakatsuhamaura, Minami-Ise, Mie 516-0193, Japan *Corresponding author: takafumi@fra.affrc.go.jp ABSTRACT: In this study, we examined the influence of water temperature on the development of herpesviral haematopoietic necrosis (HVHN) in goldfish Carassius auratus after experimentally induced infection with cyprinid herpesvirus 2 (CyHV-2). In Expt 1, Ryukin goldfish were infected with CyHV-2 by intraperitoneal injection and maintained at 4 different water temperatures. Cumulative mortalities of the 15, 20, 25 and 30°C groups were 10, 90, 90 and 60%, respectively. Therefore, the temperature range of 20-25°C is considered highly permissive for HVHN. One of 6 surviving fish of the 15°C group died after a rapid temperature increase to 25°C at 30 d post infection. All 3 Edonishiki goldfish, co-reared with the surviving Ryukin in tanks where the water temperature was increased from 15 to 25°C, died. In Expt 2, Edonishiki goldfish were exposed to CyHV-2 by bath immersion at 13 or 24°C, resulting in cumulative mortalities of 0 and 87%, respectively, at 28 d post-exposure. No mortality of the surviving Edonishiki in the 13°C treatment was observed when the water temperature was increased to 24°C. In addition, in Expt 2, no mortality was observed in any Ranchu co-reared with CyHV-2-immersed Edonishiki in the group where water temperature was increased from 13 to 24°C, even after re-immersion challenge with CyHV-2. It is interesting to note that CyHV-2 DNA was detected in the kidneys of 4 of the 5 surviving Ranchu co-reared with the CyHV-2-immersed Edonishiki group where the water temperature was increased from 13 to 24°C. Therefore, it is likely that the surviving Edonishiki of the 13°C group were virus carriers. This study indicates that most fish infected with CyHV-2 at 13-15°C acquire resistance to HVHN, but as carriers they are able to infect naïve fish. KEY WORDS: Cyprinid herpesvirus 2 · CyHV-2 · Resistance · Carassius auratus Full text in pdf format PreviousNextCite this article as: Ito T, Maeno Y (2014) Effects of experimentally induced infections of goldfish Carassius auratus with cyprinid herpesvirus 2 (CyHV-2) at various water temperatures. Dis Aquat Org 110:193-200. https://doi.org/10.3354/dao02759 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 110, No. 3. Online publication date: August 11, 2014 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2014 Inter-Research.

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