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The Molar Extinction Coefficient of Cyanmethemoglobin as Determined by Carbon Analysis

1971; Oxford University Press; Volume: 55; Issue: 2 Linguagem: Inglês

10.1093/ajcp/55.2.135

1943-7722

Harvey A. Itano, W. M. Fogarty, William C. Alford,

Electrochemical Analysis and Applications

The molar extinction coefficient of cyanmethemoglobin at 540 nm. (ϵM540) has been determined, based on the microanalytical determination of the carbon content of solutions of highly purified hemoglobin. The value of this parameter was found to be 43,500 ± 160 (mean ±SE, N= 10 samples, triplicate analysis), which is in excellent agreement with the value recently reported based on the determination of nitrogen. The internationally accepted value of the ¼ millimolar extinction coefficient of 11.0 (ϵM540 = 44,000) is slightly greater. The simultaneously determined molar extinction coefficient of cyanmethemoglobin at 281 nm. was found to be 108,000 ± 500 (mean ± SE, N = 6 samples, 16 analyses). This value is in good agreement with a theoretically derived ϵM280 of 100,280.