Portal de Periódicos da CAPES

Human bone marrow derived mesenchymal stem cells modulation by micro/nanostructured ATZ surfaces treated with femtosecond laser

2016; Frontiers Media; Volume: 4; Linguagem: Inglês

10.3389/conf.fbioe.2016.01.02423

2296-4185

Ângela Carvalho, Vilar Rui, Fernandes Maria, Monteiro Fernando,

Laser Material Processing Techniques

Event Abstract Back to Event Human bone marrow derived mesenchymal stem cells modulation by micro/nanostructured ATZ surfaces treated with femtosecond laser Angela Carvalho1, 2, 3, Rui Vilar4, Maria H. Fernandes5 and Fernando J. Monteiro1, 2, 3 1 Faculdade de Engenharia da Universidade do Porto, Departamento de Engenharia Metalúrgica e Materiais, Portugal 2 i3S - Instituto de Investigação e Inovação em Saúde, Portugal 3 INEB - Instituto de Engenharia Biomédica, Portugal 4 Instituto Superior Técnico - Universidade de Lisboa, CeFEMA-Centro de Física eEngenharia de Materiais Avançado, Portugal 5 Faculdade de Medicina Dentária da Universidade do Porto, Laboratory for Bone Metabolism and Regeneration, Portugal Introduction: ATZ (Alumina toughened Zirconia) has been recently studied for orthopedic and dental applications, as a replacement for metals, considering its well documented properties[1]. It is widely accepted that further improvement in implants can only be achieved by endowing biomaterials with improved surface functionality[2]. In this work, we used a femtosecond laser ablation technique to modify ATZ, developing surfaces structured at the micro and nanoscale levels, in a controlled and reproducible manner. The aim was to characterize these newly developed materials and evaluate the cell-surface interaction between Human bone marrow derived mesenchymal stem cells (hMSCs) and these micro/nanostructured surfaces (μATZ) and an untreated control (ATZ). Materials and Methods: A commercial powder of ATZ was used to prepare discs using a uniaxial press. The discs were pre-sintered, polished and sintered at 1500ºC. The laser treatments were performed in ambient atmosphere using an Yb:KYW chirped-pulse-regenerative amplification laser system with a radiation wavelength of 1030 nm and a pulse duration of 500 fs. The patterns were produced by the direct writing method, using a beam delivery system based on a Michelson interferometer, as described in Oliveira et al[3]. The obtained surfaces were characterized by SEM, EDS and XRD. hMSCs were cultured on both materials for 14 days. Cells metabolic activity, proliferation and morphology were evaluated at days 1, 7 and 14 of culture. Focal adhesions were observed at day 1 by CLSM. Results and Discussion: SEM images of the μATZ and ATZ surfaces are displayed in figure 1. EDS analysis detected the same elements in both surfaces - Zr, Al, O and C. The peaks obtained by XRD correspond all to alumina and the tetragonal phase of zirconia, meaning that the laser treatment didn't cause any change to the ZrO2 monoclinic phase. In terms of biological characterization, cells metabolic activity increased with time of culture for both surfaces, and at day 14 the μATZ samples exhibited a statistically significant increase. The same results were obtained for cell proliferation by DNA quantification. Cells focal adhesions on the patterned substrate were topographically organized, mainly with the microstructure, and were larger and more mature than on the control, showing a stronger bond to the respective surface. Cells morphology at days 1 and 7 are shown in figure 2. Cells on ATZ were randomly attached while μATZ modulated hMSCs adhesion and proliferation, mainly along the microtopographic features with some cells oriented according to the nanostructures. Thus, indicating that the microtextured cues had a higher influence on cells behaviour. Conclusion: While some methods for texturing ceramics have been described, few are capable of modifying hard ceramics surface and pattern it in a completely precise and reproducible manner. Currently, there is a lack of studies evaluating the biological potential of these laser treated ceramic surfaces. With this study we showed that the micro/nanostructures modulate cell attachment and proliferation and improve cell behaviour, in relation to an untreated ATZ surface. The micropatterns showed to have a higher impact on cells behaviour. This laser technique allows for surface modifications of implants with complex shapes and with expected long shelf life, showing potential to ensure long term lifespan. FEDER funds through the Programa Operacional Factores de Competitividade – COMPETE; Portuguese funds through FCT – Fundação para a Ciência e a Tecnologia in the framework of Ângela Carvalho PhD grant (SFRH/BD/87624/2012)References:[1] M.G. Faga, et al. Journal of the European Ceramic Society 32 (2012) 2113-2120.[2] M. Nikkhah, et al. Biomaterials 33 (2012) 5230-5246.[3] V. Oliveira, et al. Optics and Laser Technology 54 (2013) 428-431. Keywords: Bone Regeneration, microstructure, Surface modification, Cell modulation Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: New Frontier Oral Topic: Modeling cellular events in tissue regeneration Citation: Carvalho A, Vilar R, Fernandes MH and Monteiro FJ (2016). Human bone marrow derived mesenchymal stem cells modulation by micro/nanostructured ATZ surfaces treated with femtosecond laser. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02423 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers' terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Angela Carvalho Rui Vilar Maria H Fernandes Fernando J Monteiro Google Angela Carvalho Rui Vilar Maria H Fernandes Fernando J Monteiro Google Scholar Angela Carvalho Rui Vilar Maria H Fernandes Fernando J Monteiro PubMed Angela Carvalho Rui Vilar Maria H Fernandes Fernando J Monteiro Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.