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Identification of the Grincamycin Gene Cluster Unveils Divergent Roles for GcnQ in Different Hosts, Tailoring the l -Rhodinose Moiety

2013; American Chemical Society; Volume: 15; Issue: 13 Linguagem: Inglês

10.1021/ol401253p

1523-7060

Yun Zhang, Hongbo Huang, Qi Chen, Minghe Luo, Aijun Sun, Yongxiang Song, Junying Ma, Jianhua Ju,

Biochemical and Molecular Research

The gene cluster responsible for grincamycin (GCN, 1) biosynthesis in Streptomyces lusitanus SCSIO LR32 was identified; heterologous expression of the GCN cluster in S. coelicolor M512 yielded P-1894B (1b) as a predominant product. The ΔgcnQ mutant accumulates intermediate 1a and two shunt products 2a and 3a bearing l-rhodinose for l-cinerulose A substitutions. In vitro data demonstrated that GcnQ is capable of iteratively tailoring the two l-rhodinose moieties into l-aculose moieties, supporting divergent roles of GcnQ in different hosts.