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Comparison of Lipid and n-3 Highly Unsaturated Fatty Acid Incorporations between Artemia Enriched with Various Types of Oil by Direct Method.

1992; Springer Science+Business Media; Volume: 58; Issue: 2 Linguagem: Inglês

10.2331/suisan.58.277

1349-998X

Toshio Takeuchi, Masanori Toyota, Takeshi Watanabe,

Algal biology and biofuel production

Duplicate experiments were conducted to compare the incorporation of lipid and n-3 highly unsaturated fatty acids (n-3HUFA) in Artemia between various types of oil, such as ethyl esters (EE), triglycerides (TG), methyl esters (ME), and free fatty acids (FFA) containing 43% of n-3HUFA, using the so-called direct method. The newly-hatched nauplii of Artemia from Utah, USA were enriched with emulsions prepared with these lipids for 0, 6, 12, 24, and 48h. The lipid contents of Artemia enriched with TG, ME, and EE were higher, particularly after 12h, than those of the non-enriched and FFA-fed groups in both the experiments. The TG level increased and reached a plateau after 24h in the nauplii fed on emulsified TG, ME, and EE, sug-gesting that esters such as ME and EE were converted very quickly to TG in Artemia. Thereafter, the FFA levels in Artemia fed on emulsified oils increased inversely to the TG levels, suggesting that TG in Artemia were converted to FFA to be utilized as an energy source, particularly from around 24h after feeding. The maximum n-3HUFA levels (7-10% on a dry matter basis) were recorded for the EE enriched Artemia, whereas the variations were minimal for the non-enriched group. The levels were also low in the FFA-fed Artemia. It may be concluded that a maximal incorporation of n-3HUFA in Artemia can be obtained by enrichment with EE for 24h and that EE or ME are very quickly incorporated into TG fractions.