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Alkalinity of Gastric Venous Blood During Gastric Secretion.

1931; SAGE Publishing; Volume: 28; Issue: 6 Linguagem: Inglês

10.3181/00379727-28-5487

1535-3702

Martin Hanke, R. E. Johannesen, M. Hanke,

Diet and metabolism studies

This study was undertaken to determine whether or not gastric HC1 formation is accompanied by the simultaneous formation of an equivalent amount of alkali by the gastric tissue. The available alkali of gastric venous blood was compared with that of arterial blood simultaneously drawn during various conditions of gastric activity. The difference in available alkali in the 2 bloods is equal to the difference in bicarbonate (Δ BHCO3 = BHCO3 in gastric blood—BHCO3 in arterial blood) plus the difference in alkali bound by protein (Δ BP) at constant pH. The BHCO3 of the blood was calculated from Hasselbalch's equation, pH = pK + log BHCO3/H2CO3, by substituting the values for pH and total CO2 experimentally determined. In calculating the difference in base bound by proteins of the 2 bloods 3 proteins must be considered, proteins of serum, Ps, hemoglobin, Hb, and oxyhemoglobin HbO2. ΔBPblood = Δ BPs + ΔBHbO2 + Δ BHb. The following equations were used for calculating each of these. Δ pH in any case is pH of gastric blood—pH of arterial blood.Δ BPs = 3.4 × Δ pH serum.Δ BHb = 3.35 × mmols Hb × Δ pH cells.Δ BHbO2 = 3.60 × mmols HbO2 × Δ pH cells.Because of their relatively small capacity for combining with base, the proteins of serum were not determined but were assumed to be constant, and the equation dBPs/d pH = 3.4 was assumed for all the samples of blood worked with. The pH of the cells, used in calculation of Δ BHb, and Δ BHbO2 was obtained from the pH of the serum, using the figure of Van Slyke, Wu and McLean, giving the relation of cell and serum pH's.