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In vitro study using calvaria-derived osteogenic primary cultures for evaluation of cytotoxicity of some Ti-based alloys for biomedical applications

2016; Frontiers Media; Volume: 4; Linguagem: Inglês

10.3389/conf.fbioe.2016.01.02887

2296-4185

Donato Tatiani, Diego Correa, Cardoso Rodrigo, Rocha Lu�s, Grandini Carlos Roberto,

Orthopaedic implants and arthroplasty

Event Abstract Back to Event In vitro study using calvaria-derived osteogenic primary cultures for evaluation of cytotoxicity of some Ti-based alloys for biomedical applications Tatiani Donato1, 2, Diego Correa1, 2, Rodrigo Cardoso2, 3, Luís Rocha1, 2 and Carlos Roberto Grandini1, 2 1 Universidade Estadual Paulista, Laboratório de Anelasticidade e Biomateriais, Brazil 2 IBTN – Institute of Biomaterials, Tribocorrosion and Nanomedicine – Brazilian Branch, Brazil 3 Univerade de São Paulo, Faculdade de Odontologia de Bauru, Brazil Titanium and some of its alloys have been largely used in the production of prostheses and special medical and dental devices due to their properties, such as low Young’s modulus values, corrosion resistance and biocompatibility[1]. Provided that there are not adverse and no uncontrollable reactions are elicited in the biological system, the implanted material should be regarded as biocompatible. The in vitro cytotoxicity tests represent the initial step of the process, and could be considered as a pre-selection of those materials[2]. This in vitro study aimed to evaluate the differentiation of calvaria-derived osteogenic primary cultures supplemented with ascorbic acid (AA; 5 µg/mL)/β-glycerophosphate (βGP; 10 mM). It was analyzed the in vitro cytotoxicity as well as the differentiation of osteoblastic cells cultived on the Ti6Al4V, Ti15Zr5Mo, Ti15Zr10Mo e Ti15Zr15Mo alloys with different time periods. We have performed, to evaluate the cytotoxicity of the alloys, the assay MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenayl-2H-tetrazolium bromide) and scanning electron microscopy (SEM) with 24, 48 and 72 hours. Besides to the mineralization nodules formation was analyzed by the assay alizarin red with 15 and 30 days as well as the alkaline phosphatase activity (ALP). The present results demonstrated that all alloys studied presented no cytotoxic effects on the osteogenic cells. In addition, the nodule mineralization formation was observed, as well as a high activity of alkaline phosphatase. All of them, independently from the material, showed a central and flattened cell body and numerous and long processes. Therefore these alloys no affected the in vitro proliferation, differentiation and biomineralization of osteoblastic cells, key characteristics to bone development. CNPq (grants #481.313/2012-5, #307.279/2013-8 and 400.705/2015-0) for financial support.References:[1] Li Y, Yang C, Zhao H, Qu S, Li X, Li Y. New Developments of Ti-Based Alloys for Biomedical Applications. Materials 2014;7:1709-800.[2] ISO. Biological evaluation of medical devices - Part 5: Tests for in vitro cytotoxicity. 10993-5. Geneve, Switzerland: International Organization for Standardization; 2009. Keywords: Cell Proliferation, Extracellular Matrix, in vitro, Biocompatibility Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomaterials in mesenchymal and hematopoietic stem cell biology Citation: Donato T, Correa D, Cardoso R, Rocha L and Grandini C (2016). In vitro study using calvaria-derived osteogenic primary cultures for evaluation of cytotoxicity of some Ti-based alloys for biomedical applications. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02887 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Tatiani Donato Diego Correa Rodrigo Cardoso Luís Rocha Carlos Roberto Grandini Google Tatiani Donato Diego Correa Rodrigo Cardoso Luís Rocha Carlos Roberto Grandini Google Scholar Tatiani Donato Diego Correa Rodrigo Cardoso Luís Rocha Carlos Roberto Grandini PubMed Tatiani Donato Diego Correa Rodrigo Cardoso Luís Rocha Carlos Roberto Grandini Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.