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Differentiation between Heterotrophic and Photosynthetic Plankton by Size Fractionation, Glucose Uptake, ATP and Chlorophyll Content

1978; Wiley; Volume: 31; Issue: 3 Linguagem: Inglês

10.2307/3543663

1600-0706

Bo Riemann,

Microbial Community Ecology and Physiology

By means of a technique which included 3H-D-glucose uptake, ATP and chlorophyll analyses in different size fractions of lake water microorganisms, the bacterial and the phytoplankton biomasses were estimated from four eutrophic lakes. Short term glucose uptake experiments and chlorophyll analyses in lake water samples were used as indicators of the bacteria and the phytoplankton present in the different size fractions, whereas ATP measurements were used to quantify their respective biomasses. The bacteria comprised between 3 and 18% of total biomass (phytoplankton + bacteria) and between 50 and 90% of the bacteria were free-living. The numbers of bacteria calculated from the bacterial ATP content were 20 to 50% higher than the numbers estimated by direct counting.