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Role of P2X7 receptor in the response of phagocytes to Plasmodium chabaudi infection

2013; Frontiers Media; Volume: 4; Linguagem: Inglês

10.3389/conf.fimmu.2013.02.00073

1664-3224

Menezes Maria, Salles �rika, Cassado Alexandra, Amaral Eduardo, Da Silva Henrique, M. Maria, S Robson, D'Imp�rio Lima Maria Regina,

Immune Cell Function and Interaction

Event Abstract Back to Event Role of P2X7 receptor in the response of phagocytes to Plasmodium chabaudi infection Maria Menezes1*, Érika M. Salles1, Alexandra Cassado1, Eduardo Amaral1, Henrique B. Da Silva1, José Maria Mosig1, Robson Silva2 and Maria Regina D'Império Lima1 1 University of São Paulo, Brazil 2 Federal University of Rio de Janeiro, Brazil Malaria is characterized by intense activation of the immune system that seems to contribute to protection and pathogenesis. ATP recognition by P2X7R in immune cells is important for cell activation and death. In this study, we evaluated the effects of P2X7R-mediated signaling in phagocytes during infection with Plasmodium chabaudi. C57BL/6 and P2X7R-/- mice were infected with P. chabaudi infected red blood cells (iRBC) and clinical parameters were measured. Kinetics of phagocytic cells and cell death in the spleen of infected animals was determined by flow cytometry. In vitro assay was performed with bone marrow derived macrophages (BMDM) from both mice strains in presence of iRBC and ATP. Between days 9 and 17 post-infection, 80% of P2X7R-/- mice died, while the C57BL/6 group presented no death. Clinical manifestations and their improvement were delayed in knockout mice in comparison to wild-type. P2X7R-/- mice showed a higher number of CD11b+ cells at day 7 post-infection compared to the wild-type mice. The percentage of cell death found in spleen in the same day was lower in knockout group. Moreover, BMDM from C57BL/6 mice became significantly more sensitive to ATP when exposed previously to iRBC in a P2X7-dependent way when compared to that without iRBC. Thus, mice lacking the P2X7R seem to be more susceptible to P. chabaudi infection, presenting more severe clinical manifestations. Furthermore, the elevated number of phagocytes in spleen from P2X7R-/- mice when compared to C57BL/6 mice could be due to the reduced rate of cell death found in those cells. Acknowledgements Fapesp and CNPq Keywords: P2X7, Malaria, Phagocytes, ATP, Plasmodium Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Menezes M, Salles ÉM, Cassado A, Amaral E, Da Silva HB, Mosig J, Silva R and D'Império Lima M (2013). Role of P2X7 receptor in the response of phagocytes to Plasmodium chabaudi infection. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00073 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 08 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Maria Menezes, University of São Paulo, São Paulo, Brazil, menezesnmaria@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Maria Menezes Érika M Salles Alexandra Cassado Eduardo Amaral Henrique B Da Silva José Maria Mosig Robson Silva Maria Regina D'Império Lima Google Maria Menezes Érika M Salles Alexandra Cassado Eduardo Amaral Henrique B Da Silva José Maria Mosig Robson Silva Maria Regina D'Império Lima Google Scholar Maria Menezes Érika M Salles Alexandra Cassado Eduardo Amaral Henrique B Da Silva José Maria Mosig Robson Silva Maria Regina D'Império Lima PubMed Maria Menezes Érika M Salles Alexandra Cassado Eduardo Amaral Henrique B Da Silva José Maria Mosig Robson Silva Maria Regina D'Império Lima Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.