Produção Nacional
Revisado por pares
First Report of a New Lineage in the Fusarium solani Species Complex Causing Root Rot on Sunn Hemp in Brazil
2016; American Phytopathological Society; Volume: 100; Issue: 8 Linguagem: Inglês
10.1094/pdis-08-15-0947-pdn
ISSN1943-7692
AutoresMaruzanete Pereira de Melo, José Evando Aguiar Beserra, Kedma da Silva Matos, Cristiano S. Lima, Olinto Liparini Pereira,
Tópico(s)Banana Cultivation and Research
ResumoHomePlant DiseaseVol. 100, No. 8First Report of a New Lineage in the Fusarium solani Species Complex Causing Root Rot on Sunn Hemp in Brazil PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of a New Lineage in the Fusarium solani Species Complex Causing Root Rot on Sunn Hemp in BrazilM. P. Melo, J. E. A. Beserra Jr, K. S. Matos, C. S. Lima, and O. L. PereiraM. P. MeloSearch for more papers by this author, J. E. A. Beserra JrSearch for more papers by this author, K. S. MatosSearch for more papers by this author, C. S. LimaSearch for more papers by this author, and O. L. PereiraSearch for more papers by this authorAffiliationsAuthors and Affiliations M. P. Melo J. E. A. Beserra Jr , Departamento de Fitotecnia, Universidade Federal do Piauí-UFPI, 64049-550, Teresina, PI, Brasil K. S. Matos , Instituto Nacional de Pesquisas da Amazônia-INPA, 69060-001, Manaus, AM, Brasil C. S. Lima , Departamento de Fitotecnia, Universidade Federal do Ceará-UFC, 60356-001, Fortaleza, CE, Brasil O. L. Pereira , Departamento de Fitopatologia, Universidade Federal de Viçosa-UFV, 36570-900, Viçosa, MG, Brasil. Published Online:24 May 2016https://doi.org/10.1094/PDIS-08-15-0947-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Species of the genus Crotalaria L. are used as soil covering, green manure, and in the management of phytopathogenic nematodes. Crotalaria paulinea Schrank. plants showing wilt and root rot were identified and collected in Caucaia, Ceará, Brazil. Fifteen plants collected in a single field presented wilt symptoms. The plants showed root rot and were covered by reddish perithecia. The fungus was isolated from a cirrus of ascospores to each plant in 2% malt extract medium and the resulting culture was grown at 25°C. Carnation leaf piece agar (CLA), potato dextrose agar (PDA), and Spezieller Nährstoffarmer agar (SNA) media (Leslie and Summerell 2006) were used for morphological observations. The cultures were deposited in the Culture Collection of Phytopathogenic Fungi of the Universidade Federal Rural de Pernambuco, Recife, Brazil (CMM 1329 and CMM 1332). The anamorphs have typical white colonies of the genus Fusarium. The microconidia are unicellular and produced at the tips of long and branched monophialides, cylindrical and ellipsoid, hyaline, 6 to 18 µm long × 2 to 6 µm wide. Neither isolate produced macroconidia on SNA. Perithecia are orange to red, pyriform, with short neck, 127.9 to 187.5 × 95.7 to 112.2 µm. Asci are unitunicate, containing eight globe-like ascospores. Ascospores globose, unicellular, cylindrical, thick and rough walled, uniseriately arranged, 11.1 to 94.9 × 10.5 to 86.2 µm. The translation elongation factor (TEF1-α) and internal transcribed spacer (ITS) genes were amplified by PCR and sequenced. The nucleotide sequences were deposited in GenBank (KT365848 to KT365851). Maximum likelihood analysis was carried out using the sequences of the two genes from Fusarium sp. and other species from the Fusarium solani species complex (FSSC). Morphological characteristics indicated that the isolates are similar to F. neocosmosporiellum; however, phylogenetic analysis revealed a distinct clade (bootstrap 99%) close to F. neocosmosporiellum (≡ N. vasinfecta) and nested in the FSSC. C. paulinea seeds were used in a pathogenicity test. Agar plugs 3 mm in diameter from the Fusarium sp. were put 0.5 cm deep, and the seeds were subsequently deposited on the agar plugs and covered with a small portion of sterilized soil. Pots were kept in a greenhouse under temperatures of 25 to 32°C. The roots were evaluated 30 days after germination, and 80% of the inoculated plants showed symptoms including root system reduction, reddish roots, and darkening of internal tissues. No symptoms were observed among noninoculated plants. The fungus was reisolated from the diseased plants. There have been reports of F. neocosmoporiellum on soybean in the EUA and China (Gray et al. 1980) on peanuts in Taiwan (Huang et al. 1992), and on cowpeas, watermelon, and cotton in the United States (Smith 1899). This is the first report of a new lineage of Fusarium belonging to the FSSC causing root rot on C. paulinea.References:Gray, F. A., et al. 1980. Plant Dis. 64:321. Crossref, Google ScholarHuang, J. W., et al. 1992. Plant Pathol. 1:203. Google ScholarLeslie, J. F., and Summerell, B. A. 2006. The Fusarium Laboratory manual. Blackwell Publishing, Ames, IA. Crossref, Google ScholarSmith, E. F. 1899. Wilt disease of cotton, watermelon and cowpea (Neocosmospora nov. gen.). U. S. Department of Agriculture. G.P.O., Washington. Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 8 August 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 22 Jul 2016Published: 24 May 2016First Look: 6 Apr 2016Accepted: 21 Mar 2016 Page: 1784 Information© 2016 The American Phytopathological SocietyCited byHaematonectria haematococca (dry rot of potato)CABI Compendium, Vol. CABI CompendiumMolecular Diagnostics and Pathogenesis of Fungal Pathogens on Bast Fiber Crops18 March 2020 | Pathogens, Vol. 9, No. 3
Referência(s)