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Distinct Properties of Nucleocapsid of a Watermelon Isolate of Tomato Spotted Wilt Virus.

1993; Phytopathological Society of Japan; Volume: 59; Issue: 5 Linguagem: Inglês

10.3186/jjphytopath.59.500

1882-0484

Kaoru Hanada, Shinya Tsuda, Mitsuro KAMEYA-IWAKI, Hiroshi Tochihara,

Plant Pathogenic Bacteria Studies

Serological and physicochemical properties of nucleocapsid of tomato spotted wilt virus (TSWV) isolated from watermelon (TSWV-W) were compared with three isolates of TSWV from dahlia (TSWV-D), green pepper (TSWV-P) and tomato (TSWV-N) in Japan. Nucleocapsid of TSWV-W also had three major RNA and one rotein. Three RNA species are tentatively called as RNA 1, RNA 2 and RNA 3, from the largest to the smallest. TSWV-W contained larger RNA 3 (Mr 1.2⁄106) and larger protein (32 K) than those of the other three isolates, although nucleocapsid of TSWV-N, -P and-D contained three major RNA species (RNAs) with Mrs of 2.7×106 (RNA 1), 2.1×106 (RNA 2) and 1.0×106 (RNA 3) and one kind of protein with Mr of 30 K. Serological relationships among the isolates were analyzed by immunodiffusion tests using antisera prepared against nucleocapsids of TSWV-W and TSWV-N. Nucleocapsid of TSWV-W and-N reacted clearly with their homologous antisera but rarely or only weakly reacted with heterologous tisera. Nucleocapsid of TSWV-P and-D was serologically closely related to that of TSWV-N but not to TSWV-W. Consequently, TSWV-W was found to be unique on the basis of properties of nucleocapsid among the four TSWV isolates compared.