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The influence of lateral forces on the cell stiffness measurement by optical tweezers vertical indentation

2016; Taylor & Francis; Volume: 10; Issue: 1 Linguagem: Inglês

10.1080/15599612.2016.1149896

1559-9620

Fatou Ndoye, Muhammad Sulaiman Yousafzai, Giovanna Coceano, Serena Bonin, G. Scoles, Oumar Ka, Joseph Niemela, Dan Cojoc,

Microfluidic and Bio-sensing Technologies

We studied the lateral forces arising during the vertical indentation of the cell membrane by an optically trapped microbead, using back focal plane interferometry to determine force components in all directions. We analyzed the cell-microbead interaction and showed that indeed the force had also lateral components. Using the Hertz model, we calculated and compared the elastic moduli resulting from the total and vertical forces, showing that the differences are important and the total force should be considered. To confirm our results we analyzed cells from two breast cancer cell lines: MDA-MB-231 and HBL-100, known to have different cancer aggressiveness and hence stiffness.