Determination of Damage-free Crystal Structure of an X-ray Sensitive Protein Using XFEL
2015; The Crystallographic Society of Japan; Volume: 57; Issue: 2 Linguagem: Inglês
10.5940/jcrsj.57.122
ISSN1884-5576
AutoresKunio Hirata, Kyoko Shinzawa‐Itoh, Go Ueno, Masaki Yamamoto, Hideo Ago,
Tópico(s)Advanced X-ray Imaging Techniques
ResumoWe report a method of femtosecond crystallography for determining radiation damage-free crystal structures of large proteins at atomic resolution. The name of the method is ʻserial femtosecond rotation crystallographyʼ (SF-ROX). Here, we demonstrate experimental details of SF-ROX and 1.9-Å radiation damage-free structure analysis of bovine cytochrome c oxidase, a large (420-kDa), highly radiation-sensitive membrane protein.
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