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Effect of apoptosis on gastric adenocarcinoma cell line SGC-7901 induced by cis -9, trans -11-conjugated linoleic acid

2002; Baishideng Publishing Group; Volume: 8; Issue: 6 Linguagem: Inglês

10.3748/wjg.v8.i6.999

2219-2840

Jia‐Ren Liu,

RNA modifications and cancer

AIM:To determine the effect of apoptosis on gastric cancer cells (SGC-7901) induced by cis-9, trans-11-conjugated linoleic acid (c9, t11-CLA) and its possible mechanism in the inhibition of cancer cells growth. METHODS:Using cell culture, flow cytometery and immunocytochemical techniques, we examined the cell growth, frequency of apoptosis and distribution of cell cycle, expression of ki67, bcl-2, Fas, and c-myc of SGC-7901 cells which were treated with various c9, t11-CLA concentrations (25,50,100 and 200 µmol•L -1 ) of c9, t11-CLA for 24h and 48 h, with a negative control (0.1 % ethanol). RESULTS:The growth of SGC-7901 cells was inhibited by c9,t11-CLA.Eight days after treatment with various concentrations of c9,t11-CLA, as mentioned above, the inhibition rates were 5.9 %, 20.2 %,75.6 % and 82.4 %, respectively.The frequency of apoptosis on SGC-7901 cells induced by different concentrations of c9, t11-CLA (except for 25 µmol•L -1 , 24 h) was significantly greater than that in the negative control (P<0.01).To further investigate the influence of the cell cycle progression, we found that apoptosis induced by c9, t11-CLA may be involved in blocking the cell cycle of SGC-7901 cells.Immunocytochemical staining demonstrated that SGC-7901 cells preincubated in media supplemented with different c9, t11-CLA concentrations for various time periods significantly decreased the expressions of ki67 (the expression rates were 18.70-3.20%, at 24 h and 8.10-0.20 % at 48 h, respectively), bcl-2 (4.30-0.15% at 24 h and 8.05 %-0 at 48 h),and c-myc (4.85-2.20 % at 24 h and 4.75-0.30% at 48 h) as compared with those in the controls (the expressions of ki67, bcl-2, and c-myc were 15.1 % at 24 h and 13.5 % at 48 h, 6.80 % at 24 h and 8.00 % at 48 h, 5.50 % at 24 h and 5.30 % at 48 h, respectively) (P<0.01),whereas the expressions of Fas were increased (0.60-2.75 %, 24 h and 0.45-5.95%, 48 h). CONCLUSION:The growth and proliferation of SGC-7901 cells are inhibited by c9, t11-CLA via blocking the cell cycle, pathways of bcl-2-associated mitochondria with reduced expression of bcl-2 and Fas-associated death domain protein (FADD) with enhanced expression of Fas.But expression of c-myc on SGC-7901 cells is lower than that in negative control, which needs to be studied further.