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IL-17A promotes migration and tumor killing capability of B cells in esophageal squamous cell carcinoma

2016; Impact Journals LLC; Volume: 7; Issue: 16 Linguagem: Inglês

10.18632/oncotarget.7869

1949-2553

Lin Lü, Chengyin Weng, Haibo Mao, Xisheng Fang, Xia Liu, Yong Wu, Xiaofei Cao, Baoxiu Li, Xiaojun Chen, Qinquan Gan, Jian‐Chuan Xia, Guolong Liu,

Immunotherapy and Immune Responses

We have previously reported that the accumulation of IL-17-producing cells could mediate tumor protective immunity by promoting the migration of NK cells, T cells and dendritic cells in esophageal squamous cell carcinoma (ESCC) patients. However, there were no reports concerning the effect of IL-17A on tumor infiltrating B cells. In this study, we investigated the accumulation of CD20+ B cells in the ESCC tumor nests and further addressed the effect of IL-17A on the migration and cytotoxicity of B cells. There was positive correlation between the levels of CD20+ B cells and IL-17+ cells. IL-17A could promote the ESCC tumor cells to produce more chemokines CCL2, CCL20 and CXCL13, which were associated with the migration of B cells. In addition, IL-17A enhanced the IgG-mediated antibody and complement mediated cytotoxicity of B cells against tumor cells. IL-17A-stimulated B cells gained more effective direct killing capability through enhanced expression of Granzyme B and FasL. The effect of IL-17A on the migration and cytotoxicity of B cells was IL-17A pathway dependent, which could be inhibited by IL-17A inhibitor. This study provides further understanding of the roles of IL-17A in humoral response, which may contribute to the development of novel tumor immunotherapy strategy.