Survival behaviour and virulence of the fish pathogen Vibrio ordalii in seawater microcosms
2016; Inter-Research; Volume: 120; Issue: 1 Linguagem: Inglês
10.3354/dao03005
ISSN1616-1580
AutoresPamela Ruíz, Matías Poblete‐Morales, Rute Irgang, AE Toranzo, Rubén Avendaño‐Herrera,
Tópico(s)Identification and Quantification in Food
ResumoDAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 120:27-38 (2016) - DOI: https://doi.org/10.3354/dao03005 Survival behaviour and virulence of the fish pathogen Vibrio ordalii in seawater microcosms Pamela Ruiz1,2, Matías Poblete-Morales1,2,3, Rute Irgang1,2, Alicia E. Toranzo4, Ruben Avendaño-Herrera1,2,3,* 1Laboratorio de Patología de Organismos Acuáticos y Biotecnología Acuícola, Facultad de Ciencias Biológicas, Universidad Andrés Bello, 2520000 Viña del Mar, Chile 2Interdisciplinary Center for Aquaculture Research (INCAR), 4030000 Concepción, Chile 3Centro de Investigación Marina Quintay (CIMARQ), Universidad Andres Bello, Quintay, Chile 4Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain *Corresponding author: reavendano@yahoo.com ABSTRACT: Vibrio ordalii, the causative agent of atypical vibriosis, is a Gram-negative, motile, rod-shaped bacterium that severely affects the salmonid aquaculture industry. V. ordalii has been biochemically, antigenically and genetically characterized. However, studies on the survival behaviour of this bacterium in aquatic environments are scarce, and there is no information regarding its disease transmission and infectious abilities outside of the fish host or regarding water as a possible reservoir. The present study investigated the survival behaviour of V. ordalii Vo-LM-06 and Vo-LM-18 in sterile and non-sterile seawater microcosms. After a year in sterile seawater without nutrients, 1% of both V. ordalii strains survived (~103 colony-forming units ml-1), and long-term maintenance did not affect bacterial biochemical or genetic properties. Additionally, V. ordalii maintained for 60 d in sterile seawater remained infective in rainbow trout Oncorhynchus mykiss. However, after 2 d of natural seawater exposure, this bacterium became non-culturable, indicating that autochthonous microbiota may play an important role in survival. Recuperation assays that added fresh medium to non-sterile microcosms did not favour V. ordalii recovery on solid media. Our results contribute towards a better understanding of V. ordalii survival behaviour in seawater ecosystems. KEY WORDS: Vibrio ordalii · Vibriosis · Survival · Microcosm Full text in pdf format PreviousNextCite this article as: Ruiz P, Poblete-Morales M, Irgang R, Toranzo AE, Avendaño-Herrera R (2016) Survival behaviour and virulence of the fish pathogen Vibrio ordalii in seawater microcosms. Dis Aquat Org 120:27-38. https://doi.org/10.3354/dao03005 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 120, No. 1. Online publication date: June 15, 2016 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2016 Inter-Research.
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