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GTP interaction with a protein synthesis initiation factor preparation from Escherichiacoli

1967; Elsevier BV; Volume: 28; Issue: 1 Linguagem: Inglês

10.1016/0006-291x(67)90410-x

1090-2104

Jorge E. Allende, Herbert Weissbach,

RNA and protein synthesis mechanisms

This chapter discusses that protein synthesis is a complex process involving multiple steps and requiring numerous cellular components. It reviews that the role of GTP in protein synthesis is the subject of numerous studies and speculations. It discusses the structure of the E. coli ribosome that provides insight into the ribosomal mechanisms at the molecular level. The translocation step as a central event of the ribosomal elongation process still remains as obscure as ever. Nonenzymic polypeptide synthesis may yield a suitable and simple assay system for investigating the interactions among tRNA, mRNA, and ribosomal components during translocation. The mechanism of elongation in eukaryotes is, in many ways, similar to the one in prokaryotes. Thus far, only EF-1 has proved to differ from the corresponding factor EF-T. The chapter also provides an overview on the eukaryotic elongation mechanism, its structure and mechanism of action of EF-1.