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Chromosome Banding in Crustacea. I. Karyotype, Ag-NORs, C Banding and Treatment with EcoRI, PstI and KpnI Restriction Endonucleases in Artemia franciscana

2014; UNIVERSIDADE FEDERAL DO AMAPÁ; Volume: 4; Issue: 2 Linguagem: Inglês

10.18561/2179-5746/biotaamazonia.v4n2p15-19

ISSN

2179-5746

Autores

Ingrid Vilar Accioly, Inailson Márcio Costa da Cunha, Joana Cristina Medeiros Tavares, Wagner Franco Molina,

Tópico(s)

Protist diversity and phylogeny

Resumo

Karyotypic characteristics of the microcrustacean Artemia franciscana Kellog 1906, introduced to the salt lakes on the northeastern coast of Brazil in the 1970s, were investigated by conventional staining, C banding, restriction endonucleases (EcoRI, PstI and KpnI) and Ag-NORs. The karyotype consisted of 42 chromosomes and secondary constrictions were observed on some pairs. Large heterochromatic blocks were found distributed in the telomere portion of most of the chromosomes. Digestion with PstI and KpnI showed a similar pattern to that obtained by C banding. Preparations treated with EcoRI revealed intense action in the heterochromatic regions indicating the presence of restriction sites. Multiple Ag-NORs were shown associated to heterochromatic blocks. These data presented here no identified modifications that might have occurred after the geographic isolation of this stock and examine the evolutionary modifications in the karyotype of this group. Keywords : Chromosome banding; brine shrimp; crustacean cytogenetics.

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