rnaQUAST: a quality assessment tool for de novo transcriptome assemblies
2016; Oxford University Press; Volume: 32; Issue: 14 Linguagem: Inglês
10.1093/bioinformatics/btw218
ISSN1367-4811
AutoresElena Bushmanova, Dmitry Antipov, Alla Lapidus, В. Д. Суворов, Andrey D. Prjibelski,
Tópico(s)RNA modifications and cancer
ResumoAbility to generate large RNA-Seq datasets created a demand for both de novo and reference-based transcriptome assemblers. However, while many transcriptome assemblers are now available, there is still no unified quality assessment tool for RNA-Seq assemblies. We present rnaQUAST-a tool for evaluating RNA-Seq assembly quality and benchmarking transcriptome assemblers using reference genome and gene database. rnaQUAST calculates various metrics that demonstrate completeness and correctness levels of the assembled transcripts, and outputs them in a user-friendly report.rnaQUAST is implemented in Python and is freely available at http://bioinf.spbau.ru/en/rnaquastap@bioinf.spbau.ruSupplementary data are available at Bioinformatics online.
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