Quantitation of Muscle Glycogen Synthesis in Normal Subjects and Subjects with Non-Insulin-Dependent Diabetes by 13 C Nuclear Magnetic Resonance Spectroscopy
1990; Massachusetts Medical Society; Volume: 322; Issue: 4 Linguagem: Inglês
10.1056/nejm199001253220403
ISSN1533-4406
AutoresGerald I. Shulman, Douglas L. Rothman, Thomas Jue, Peter Stein, Ralph A. DeFronzo, Robert G. Shulman,
Tópico(s)Adipose Tissue and Metabolism
ResumoTo examine the extent to which the defect in insulin action in subjects with non-insulin-dependent diabetes mellitus (NIDDM) can be accounted for by impairment of muscle glycogen synthesis, we performed combined hyperglycemic—hyperinsulinemic clamp studies with [13C]glucose in five subjects with NIDDM and in six age- and weight-matched healthy subjects. The rate of incorporation of intravenously infused [1–13C]glucose into muscle glycogen was measured directly in the gastrocnemius muscle by means of a nuclear magnetic resonance (NMR) spectrometer with a 15.5-minute time resolution and a 13C surface coil.
Referência(s)