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BIBTEX RIS

Whole-genome optical mapping reveals a mis-assembly between two rRNA operons of Corynebacterium pseudotuberculosis strain 1002

2016; BioMed Central; Volume: 17; Issue: 1 Linguagem: Inglês

10.1186/s12864-016-2673-7

ISSN

1471-2164

Autores

Diego Mariano, Thiago de Jesus Sousa, Felipe Luiz Pereira, Flávia Figueira Aburjaile, Debmalya Barh, Flávia Rocha, Anne Cybelle Pinto, Syed Shah Hassan, Tessália Diniz Luerce Saraiva, Fernanda Alves Dorella, Alex F. Carvalho, Carlos Augusto Gomes Leal, Henrique César Pereira Figueiredo, Artur Silva, Rommel Thiago Jucá Ramos, Vasco Azevedo,

Tópico(s)

Bacterial Identification and Susceptibility Testing

Resumo

Studies have detected mis-assemblies in genomes of the species Corynebacterium pseudotuberculosis. These new discover have been possible due to the evolution of the Next-Generation Sequencing platforms, which have provided sequencing with accuracy and reduced costs. In addition, the improving of techniques for construction of high accuracy genomic maps, for example, Whole-genome mapping (WGM) (OpGen Inc), have allow high-resolution assembly that can detect large rearrangements.In this work, we present the resequencing of Corynebacterium pseudotuberculosis strain 1002 (Cp1002). Cp1002 was the first strain of this species sequenced in Brazil, and its genome has been used as model for several studies in silico of caseous lymphadenitis disease. The sequencing was performed using the platform Ion PGM and fragment library (200 bp kit). A restriction map was constructed, using the technique of WGM with the enzyme KpnI. After the new assembly process, using WGM as scaffolder, we detected a large inversion with size bigger than one-half of genome. A specific analysis using BLAST and NR database shows that the inversion occurs between two homology RNA ribosomal regions.In conclusion, the results showed by WGM could be used to detect mismatches in assemblies, providing genomic maps with high resolution and allow assemblies with more accuracy and completeness. The new assembly of C. pseudotuberculosis was deposited in GenBank under the accession no. CP012837.

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