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Effect of 360His mutation in apolipoprotein A-IV on plasma HDL-cholesterol response to dietary fat

1997; Elsevier BV; Volume: 38; Issue: 10 Linguagem: Inglês

10.1016/s0022-2275(20)37130-3

1539-7262

S Jansen, José López‐Miranda, José M. Ordovás, José Luis Zambrana, Carmen Marı́n, M. Ostos, Pedro Castro, Ruth McPherson, Fernando Segura, A Blanco, J A Jiménez Perepérez, Francisco Pérez‐Jiménez,

Diet, Metabolism, and Disease

In order to determine whether genetic variability of apolipoprotein (apo) A-IV is responsible for the improvement in lipid profile when dietary saturated fats are replaced by carbohydrates or monounsaturated fats, 41 healthy male subjects were studied: 33 were homozygous for the 360Gln allele and 8 were heterozygote carriers of the 360His allele.These were administered three consecutive 4-week diets.The first was a diet rich in saturated fat (SAT diet, with 38% fat, 20% saturated.This was followed by a low fat diet (NCEP-I, with < 30% fat, < 10% saturated).The final diet was rich in monounsaturated fat (MUFA diet, with 38% fat, 22% monounsaturated).There was no difference in plasma lipid and apolipoprotein levels of both groups of individuals after consuming the SAT diet.Switching from this diet to the NCEP-I diet, carriers of the 360His allele presented a greater decrease in high density lipoprotein-cholesterol (HDL-C) (-10 vs. -1 mg/dL, P < 0.004) and apoA-I levels (-19 vs. -8 mg/ dL, P < 0.037).Similarly, replacement of carbohydrates by monounsaturated fats produced a greater increase in HDL-C (9 vs. 1 mg/dL, P < 0.003) and apoA-I levels (9 vs. 2 mg/ dL, P < 0.036) in carriers of the 360His mutation.Lecithin: cholesterol acyltransferase (LCAT) and cholesteryl ester transfer protein (CETP) activities and apoA-IV levels were also measured.However, no genotype-related differences were observed for these parameters.I Our results suggest that variability in HDL-C and apoA-I response to diet is, at least partially, determined by the 360His mutation of apoA-IV.