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Characterization of microsatellites identified by next‐generation sequencing in the Neotropical tree Handroanthus billbergii (Bignoniaceae)

2016; Botanical Society of America; Volume: 4; Issue: 5 Linguagem: Inglês

10.3732/apps.1500135

2168-0450

Eduardo Morillo, Johanna Buitrón, Ricardo Limongi, Hélène Vignes, Xavier Argout,

Genomics and Phylogenetic Studies

Premise of the study: We developed microsatellite (simple sequence repeat [SSR]) markers in the Neotropical tree Handroanthus billbergii (Bignoniaceae), to be applied in assessment of genetic diversity in this species as a reference for inferring the impact of dry forest fragmentation in Ecuador. Methods and Results: Using next‐generation sequencing, we detected a total of 26,893 putative SSRs reported here. Using an ABI 3500xl sequencer, we identified and characterized a set of polymorphic markers in 23 individuals belonging to three populations of H. billbergii . Conclusions: We report a set of 30 useful SSR markers for H. billbergii and a large list of potential microsatellites for developing new markers for this or related species.