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Molecular Identification of Cactus virus X Infecting Hylocereus polyrhizus (Cactaceae) in Hainan Island, China

2016; American Phytopathological Society; Volume: 100; Issue: 9 Linguagem: Inglês

10.1094/pdis-01-16-0048-pdn

1943-7692

Chaochao Peng, Naitong Yu, Zhonglai Luo, Hongyuan Fan, Fan He, Xiong Li, Z. L. Zhang, Z. X. Liu,

Plant and animal studies

HomePlant DiseaseVol. 100, No. 9Molecular Identification of Cactus virus X Infecting Hylocereus polyrhizus (Cactaceae) in Hainan Island, China PreviousNext DISEASE NOTES OPENOpen Access licenseMolecular Identification of Cactus virus X Infecting Hylocereus polyrhizus (Cactaceae) in Hainan Island, ChinaC. Peng, N. T. Yu, Z. W. Luo, H. Y. Fan, F. He, X. H. Li, Z. L. Zhang, and Z. X. LiuC. PengSearch for more papers by this author, N. T. YuSearch for more papers by this author, Z. W. LuoSearch for more papers by this author, H. Y. FanSearch for more papers by this author, F. HeSearch for more papers by this author, X. H. LiSearch for more papers by this author, Z. L. ZhangSearch for more papers by this author, and Z. X. LiuSearch for more papers by this authorAffiliationsAuthors and Affiliations C. Peng , Institute of Tropical Fruit Trees, Hainan Academy of Agricultural Sciences, Haikou, Hainan 571100, P.R. China N. T. Yu , Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, P.R. China Z. W. Luo H. Y. Fan F. He X. H. Li Z. L. Zhang , Institute of Tropical Fruit Trees, Hainan Academy of Agricultural Sciences / Investigation Station of Tropical Fruit Trees, Ministry of Agriculture, Haikou, Hainan 571100, P.R. China Z. X. Liu , Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, P.R. China. Published Online:8 Jul 2016https://doi.org/10.1094/PDIS-01-16-0048-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Hylocereus polyrhizus Britt. & Rose (Cactaceae), commonly known as pitaya or dragon fruit, is widely cultured in tropical and subtropical regions of China and has become an increasingly important fruit in recent years. In October 2014, in a survey of diseases on pitaya, approximately 44% of field plants showing virus-like symptoms including chlorotic halos and small chlorotic spots on stems were observed in Qionghai, Hainan Island, China. Flexuous filamentous virus particles of about 600 nm in length were observed from stem-sap preparation of four symptomatic plants with a transmission electron microscope (TEM). To further ascertain the presence of Cactus virus X (CVX), stems from 20 symptomatic samples (including four samples found positive by TEM) and 20 healthy samples were collected from different fields for RT-PCR. Total RNAs were extracted and reverse transcribed using random primers according to the manufacturer’s instructions for the RevertAid first strand cDNA synthesis kit (Thermo Scientific). The cDNA of each sample was then used in PCR using the CVX-specific forward (5′-CCCTCTATGTTTCTTACCTATTTG-3′) and reverse (5′-CTACTGCGGGAGCCTTGTTC-3′) primers designed based on a conserved sequence spanning the triple gene block protein 3 (TGB-3) and coat protein (CP) genes (Accession No. AF308158). A fragment of approximately 900 bp was amplified from each symptomatic sample. No amplification was observed when cDNA from healthy plant samples was used as a template. The PCR products were cloned into the pMD18-T vector (Takara, Dalian, China) and sequenced at ThermoFisher Scientific in Shanghai, China. The sequences were deposited in GenBank (Accession Nos. KU497493 and KU497494, respectively). Both nucleotide sequences were analyzed by BLASTn and found to share 97% identity with a Taiwan isolate (KM365479) that was reported from Hylocereus undatus Britt. & Rose (Liou et al. 2001). The amino acid sequences of the coat protein showed 97.1 and 99.1% identity with a South Korean isolate (BAO73885) by BLASTp. Phylogenetic analysis of the CP amino acid sequences using MEGA6.0 (Tamura et al. 2013) showed that Hainan isolates and all other CVX isolates formed a high-confidence subclade, except AFJ00097 (Taiwan-NTU). To our knowledge, this is the first report of CVX on H. polyrhizus Britt. & Rose in Hainan Island, China. The presence of this destructive virus on pitaya could represent a serious threat to this fruit, and therefore further surveys are needed from more tropical and subtropical areas of China in order to determine its prevalence and yield losses on pitaya.References:Liou, M. R., et al. 2001. Plant Dis. 85:229. https://doi.org/10.1094/PDIS.2001.85.2.229A Link, Google ScholarTamura, K., et al. 2013. Mol. Biol. Evol. 30:2725. https://doi.org/10.1093/molbev/mst197 Crossref, ISI, Google ScholarDetailsFiguresLiterature CitedRelated Vol. 100, No. 9 September 2016SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 22 Aug 2016Published: 8 Jul 2016First Look: 2 May 2016Accepted: 25 Apr 2016 Page: 1956 Information© 2016 The American Phytopathological SocietyCited byFirst report of cactus virus X in Spain4 October 2022 | Australasian Plant Disease Notes, Vol. 17, No. 1First record of Cactus virus X in Dragon Fruit (Hylocereus spp.) in India7 September 2021 | Indian Phytopathology, Vol. 75, No. 1A brief review of plant diseases caused by Cactus virus XCrop Protection, Vol. 143Complete genome sequence of a new member of the genus Badnavirus from red pitaya (Hylocereus polyrhizus)7 February 2020 | Archives of Virology, Vol. 165, No. 3Diseases of dragon fruit (Hylocereus species): Etiology and current management optionsCrop Protection, Vol. 126Hylocereus spp. (Hylocereus undatus; H. polyrhizus) (Pitahaya)6 June 2020First Report of Cactus virus X in Hylocereus undatus (Dragon Fruit) in FloridaR. 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