The promoting action of chaperonin CpkB on the renaturation of denatured nrhTNF in vitro
1999; Fourth Military University; Linguagem: Inglês
ISSN
1000-2790
Autores Tópico(s)Protein Structure and Dynamics
ResumoAIM: To study the possibility of using heat shockprotein CpkB from hyperthermophilic bacteria KODl to promote the renaturation of denatured recombinant new humantumor necrosis factor (nrhTNF) in vitro. METHODS: nrhTNF was denatured in mol/l. Urea and then renatured in different concentrations of CpkB expressed and purifired fromE. colt JM 109 (pBVCpk ). The percentage of renaturationwas measured by the detection of nrhTNF activity in I-929cell line. RESULTS: CpkB, as a monomer, promoted the renaturatlon of nrhTNF when the ratio of CpkB to nrhTNF is10: I -- 25: l, the highest percentage of renaturation beingup to 75 in the experiment. While in negative control, thepercentage of renaturation is only 31. CONCLUSION: CpkBhas been proved to be a molecular chaperone, which can bindmalfolded protein and mediate the correct assembly ofpolypeptlde. CpkB can be applied in protein renaturation invitro.
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