Pharmacokinetics of Florfenicol in Pelodiscus sinensis Japanese Strain
2013; Institute of Zoology, Chinese Academy of Sciences; Linguagem: Inglês
ISSN
0250-3263
Autores Tópico(s)Analytical Chemistry and Sensors
ResumoPharmacokinetics of florfenicol were studied in tissues from Pelodiscus sinensis Japanese strain using high performance liquid chromatography-tandem mass spectrometry.One hundred fifty healthy soft-shell turtles with the weight of 250±50 g were adnubustered florfenicol at a dosage of 30 mg/kg for 7 consecutive days under the temperature of 25.0±2.0℃.After the last feeding,tissues including blood,muscle,liver and kidney were collected at 1 h,2 h,4 h,8 h,16 h,24 h,48 h,72 h,96 h,120 h,144 h,168 h,240 h and 360 h,respectively.Florfenicols in different tissues were extracted with ethyl acetate,cleaned by hexane,and detected by HPLC-MS/MS with negative electrospray ionization under multiple reaction monitoring.Quantitative analysis was used by internal standard method.Results showed that: The peak times of concentrations of florfenicol in plasma,muscle,liver and kidney were 4 h,8 h,8 h and 8 h,with the maximum content of 167 μg/kg,188 μg/kg,67.15 μg/kg and 85.71 μg/kg,respectively.The elimination half life was14.9 h,9.4 h,29.5 h and 13.0 h.The concentration-time curves of florfenicol in blood,liver,muscle and kidney were: Cblood=210.332 e-0.070 6 t+938.161 e-0.046 5 t,Cmuscle=5 642.635 e-0.077 1 t+5 765.891 e-0.074 4 t,Cliver=111.596 e-0.036 0 t+4.339 e-0.023 5 t and Ckidney=176.509 e-0.0654 t+615.697 e-0.053 6 t,respectively.Florfenicol was absorbed and eliminated fast in P.sinensis Japanese strain.The withdraw period should not be less than 15 d under this experiment condition.
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