Study on Tissue Culture Seedlings of Polysics balfouriana
2010; Chinese Academy of Agricultural Sciences; Volume: 38; Issue: 15 Linguagem: Inglês
ISSN
0517-6611
Autores Tópico(s)Plant-Derived Bioactive Compounds
Resumo[Objective] The research aimed to study the tissue culture seedlings of Polysics balfouriana.[Method] Soft stems were used as explants and lots of medium was used to study the tissue culture regeneration potential of Polysics balfouriana.[Result] The soft stems of Polysics balfouriana sterilized with 0.1% hydrargyrum for 8 to 10 minutes were suitable for explants.The best medium for inducing callus was MS+BA 3.0 mg/L+NAA 0.5 mg/L,and the inducing ratio reached 85.6%.The best medium for shoot regeneration was MS+BA 1.5 mg/L+NAA 0.1 mg/L.When the shoots were cultured on a medium of 1/2MS+IBA 1.0 mg/L,more than 94.9% rooted and survived to develop thick shoots.The best potting media for subsequent ex-vitro development of the tissue culture plantlets comprised 2∶1 of peat and composted coconut powder.[Conclusion] This study provided references for tissue culture seedlings of Polysics balfouriana.
Referência(s)