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Genomic Characterization and Pathogenicity of Iridovirus Isolated from Pearl Gourami (Trichogaster leeri)

2006; Volume: 1; Issue: 3 Linguagem: Inglês

2383-5400

Ho Yeoul Kim, Joon Bum Jeong, Lyu Jin Jun, So Hye Yoon, Hye Jin Cho, Hyun Do Jeong,

Bacteriophages and microbial interactions

In the survey of the samples infected by iridovirus, we found the natural outbreak of iridovirus disease in the ornamental fish, pearl gourami (Trichogaster leeri) in Korea. It was characterized by the appearance of enlarged cells and necrosis in the observation of the imprinted spleen cells under iridovirus. To determine the infection of iridovirus more accurately, PCR using 2 different primer sets for MCP and ATPase gene used for the diagnosis of iridovirus infection was done and found the produced fragments matched with the size of expectation. Partially determined nucleotide sequences of the MCP gene of the iridovirus isolated in this study showed very high homology (99.6%) with that of ISKNV as a reference strain. In challenge experiment with the iridovirus isolated from the moribund of pearl gourami, the pathogenicity of the isolated iridovirus was confirmed and suggested the potential of the risk associated the transfer of iridovirus from the ornamental fishes to the marine culturing fishes.