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Detection and analysis of 21 HPV-DNA types in cervical lesions in different age

2007; Elsevier BV; Linguagem: Inglês

2590-2938

Gao Lan-xang,

Cervical Cancer and HPV Research

Purpose To investigate the detection of 21 types of HPV and its significance in cervical lesions of different age groups.Methods 71 cases,aged from 20 to 64 years,of cervical lesions including hyperplasia and degeneration of squamous epithelium,condyloma acuminata,low grade CIN,and high grade CIN,were involved in this study.HPV-DNAs including 15 types of high risk and 6 types of low risk were detected by HybriMax gene arrays and flowthrough hybridization.Results 50(69%) of 71 cases were positive.The ratio of infection among different age group was the highest in the age range from 20 to 25 years,and the lowest in the age range from 31 to 40 years(P=0.286).The rate of high risk type and mixed high/low risk type was 62%,while infection rate of low risk type only was 7%.The rates of HPV infection in hyperplasia,condyloma acuminata,low grade CIN,and high grade CIN were 33%,92%,89% and 100%, respectively(χ2=26.874,P=0.001).The infected HPV types in high grade CIN were all of high risk,which were types16,33,52,and 18 in sequence, while these in low grade CIN were types 16,31,18,52,33,6,and 11.The infection rate of one HPV type only was 65%,whereas that of mixture types was 34%.Conclusions HPV infection is detected in any age groups,especially in young women,most of that do not have any lesions in the cervix and may eliminate spontaneously.The infection is correlated with lesion types significantly,and the infected rate or rate of high-risk HPV infection increases with grading of the cervical lesions.The HPV infection types in high grade CIN are types 16,33,52,and 18 in sequence,and those in low grade CIN are types 16,31,18,52 in turn.