Differential display of hepatic mRNAs regulated by dietary zinc sources
2004; Linguagem: Inglês
ISSN
0529-5130
Autores Tópico(s)Molecular Biology Techniques and Applications
ResumoAvine broilers were used to study the effect of different forms of zinc on animals with differential display reverse transcription PCR.The results showed that it was an effective way to identify genes associated with different zinc sources by silver staining mRNA differential display reverse PCR.We got 26 bands from DDRT-PCR,3 bands belonging to the group of zinc deficiency; 6 bands belonging to group of zinc sulfate and 13 bands belonging to the group of Zinc-methioneine; the other 3 bands were occupied by the both groups in which different forms of zinc were used though it is still necessary for these bands to be identified further with Northern blotting,subcloning and sequencing.
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