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Epidemiology investigation of Blastocystis hominis in Huainan areas

2002; Fourth Military University; Linguagem: Inglês

1000-2790

Chao Li,

Toxoplasma gondii Research Studies

AIM To investigate whether there exists the in fectio of Blastocystis hominis (B.h) in Huainan, Anhui Province. METHODS The fresh faeces taken from children (100), pupils (100), middle students (100) and the patients with diarrhea (403) were smeared by iodine stain and HE stain for the detection of B.h . After preliminary direct microscopy, the shape and size of B.h were detected by high power lens. The cellular immune function of the patients was detected by Biotin streptavidin (BSA). RESULTS The positive rates of B.h in fresh feces taken from the children, pupils, middle school students and the patients with diarrhea, were 1.0%(1/100), 1.0%(1/100), 0(0/100) and 5.96% (24/403) respectively. The positive rate of B.h was higher in patients with diarrhea than that in normal controls ( P 0.01). Among them, the positive rates of the patients with mild diarrhea, woderate diarrhea, severe diarrhea and obstinate diarrhea were 6.03%(14/232), 2.25%(2/89), 0(0/17) and 12.31%(8/65) respectively. The positive rates of B.h in fresh faeces of male and female were 7.52%(17/226), 3.95%(7/177), and the positive rates of B.h in urban and rural areas were 4.56%(11/241), 8.02%(13/162) respectively, there being significant difference between them ( P 0.05). The positive rates of CD3 +, CD4 +, CD8 +, CD4 +/CD8 + in blood of the people with positive B.h and negative B.h were 0.64±0.06, 0.44±0.06, 0.28±0.04, 1.53± 0.34 and 0.60±0.05, 0.40±0.05, 0.30±0.05, 1.27± 0.22 respectively, there being significant difference between the two groups ( P0.05, P 0.01). CONCLUSION B.h is a new pathogen of diarrhea in feces of patients in Huainan. It is more commonly seen in the people with chronic diarrhea and obstinate diarrhea, and is associated with the cellular immune function. The main thereunder for identified diagnosis of the disease depends on the detection of B.h in fresh feces.