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Effects of oxygen tension on neural cell development: in vitro and in vivo assays on differentiation of NE-4C neural stem cells

2009; Frontiers Media; Volume: 3; Linguagem: Inglês

10.3389/conf.neuro.01.2009.04.049

1662-5137

Emı́lia Madarász,

Pluripotent Stem Cells Research

Event Abstract Back to Event Effects of oxygen tension on neural cell development: in vitro and in vivo assays on differentiation of NE-4C neural stem cells Kornél Demeter1*, Anita Zadori1, Linda Varady1, Nóra Hádinger1, Viktor A. Agoston2, 3, Anna Gobl3, Zoltán Nagy2, Timea Kohidi1 and Emilia Madarasz1 1 Institute of Experimental Medicine, Semmelweis University , Hungary 2 Department Section of Vascular Neurology, Semmelweis University , Hungary 3 Hyperbaric Centre, Hungary Previously we demonstrated that the host environment modified the fate of stem cells implanted into the mouse forebrain. Brain lesions were shown to influence the survival and differentiation of stem cells. Therefore, we investigated the fate of GFP-4C neural stem cells in vitro and in vivo environments with different oxygen tensions. The consequences of hyperbaric oxygen (HBO) treatment (hyperoxia) were studied in vivo, in cold lesioned and intact host animals implanted with GFP-4C cells. The animals were exposed to hyperbaric oxygen (2 bar abs. for 90 minutes) on each day of the post-surgical week and were sacrificed 2, 4 or 6 weeks after the implantation. We examined the survival, differentiation and apoptotic death of implanted stem cells and the alterations in the volume of the grafts. The effects of low oxygen tension (hypoxia) were investigated on neural stem cells and their differentiating progenies, in vitro. Cell viability, the rate of neuron formation and apoptosis, as well as the gene expression patterns were compared between hypoxic (1% O2, 5% CO2, 94% N2) and normoxic cultures. The results demonstrate that hypoxic conditions do not affect the viability of non-committed neural stem cells, but modify the viability of committed neuronal precursors. Hyperbaric oxygen treatment results in an enhanced intracerebral survival and decreased proliferation of stem cells grafted into lesioned brain areas. These results suggest that oxygen tension appreciably influence survival, differentiation and gene expression of neural stem cells. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Developmental neurobiology and subcortical functions Citation: Demeter K, Zadori A, Varady L, Hádinger N, Agoston VA, Gobl A, Nagy Z, Kohidi T and Madarasz E (2009). Effects of oxygen tension on neural cell development: in vitro and in vivo assays on differentiation of NE-4C neural stem cells. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.049 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Feb 2009; Published Online: 27 Feb 2009. * Correspondence: Kornél Demeter, Institute of Experimental Medicine, Semmelweis University, Budapest, Hungary, demeter@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Kornél Demeter Anita Zadori Linda Varady Nóra Hádinger Viktor A Agoston Anna Gobl Zoltán Nagy Timea Kohidi Emilia Madarasz Google Kornél Demeter Anita Zadori Linda Varady Nóra Hádinger Viktor A Agoston Anna Gobl Zoltán Nagy Timea Kohidi Emilia Madarasz Google Scholar Kornél Demeter Anita Zadori Linda Varady Nóra Hádinger Viktor A Agoston Anna Gobl Zoltán Nagy Timea Kohidi Emilia Madarasz PubMed Kornél Demeter Anita Zadori Linda Varady Nóra Hádinger Viktor A Agoston Anna Gobl Zoltán Nagy Timea Kohidi Emilia Madarasz Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.