Construction of highly expressed eukaryotic plasmid containing NDV F gene
2003; Northwest A&F University; Linguagem: Inglês
ISSN
1671-9387
Autores Tópico(s)Animal Disease Management and Epidemiology
ResumoThe recombinant plasmid PC4F that contains the gene of protective antigen F of NDV was constructed by method of the PCR and restricted enzyme.The F geng was inserted into the downstream promotor of pcDNA4.0/His Max vetor.The result shows that we successfully get a DNA vaccine of NDV F gene that can be hightly expressed in the eukaryotic cell in order to study the mechanism and effect of the DNA vaccine.
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