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Molecular insights into the OGG1 gene, a cancer risk modifier in BRCA1 and BRCA2 mutations carriers

2016; Impact Journals LLC; Volume: 7; Issue: 18 Linguagem: Inglês

10.18632/oncotarget.8272

1949-2553

Carlos Benítez‐Buelga, Tereza Vaclová, Sofia Ferreira, Miguel Urioste, Lucía Inglada‐Pérez, Nora Soberón, Marı́a A. Blasco, Ana Osório, Javier Benı́tez,

CRISPR and Genetic Engineering

// Carlos Benitez-Buelga 1 , Tereza Vaclová 1 , Sofia Ferreira 1 , Miguel Urioste 2, 5 , Lucia Inglada-Perez 3, 5 , Nora Soberón 4 , Maria A. Blasco 4 , Ana Osorio 1, 5 , Javier Benitez 1, 5 1 Human Genetics Group, Spanish National Cancer Research Center (CNIO), Madrid 28029, Spain 2 Familial Cancer Clinical Unit, Spanish National Cancer Research Center (CNIO), Madrid 28029, Spain 3 Endocrine Cancer Group, Spanish National Cancer Research Center (CNIO), Madrid 28029, Spain 4 Telomere and Telomerase Group, Spanish National Cancer Research Center (CNIO), Madrid 28029, Spain 5 Spanish Network on Rare Diseases (CIBERER), Madrid 28029, Spain Correspondence to: Javier Benitez, e-mail: jbenitez@cnio.es Keywords: BRCA1 and BRCA2, telomere shortening, OGG1 polymorfism, cancer risk modifier, DNA damage Received: January 28, 2016 Accepted: March 07, 2016 Published: March 22, 2016 ABSTRACT We have recently shown that rs2304277 variant in the OGG1 glycosidase gene of the Base Excision Repair pathway can increase ovarian cancer risk in BRCA1 mutation carriers. In the present study, we aimed to explore the role of this genetic variant on different genome instability hallmarks to explain its association with cancer risk. We have evaluated the effect of this polymorphism on OGG1 transcriptional regulation and its contribution to telomere shortening and DNA damage accumulation. For that, we have used a series of 89 BRCA1 and BRCA2 mutation carriers, 74 BRCAX cases, 60 non-carrier controls and 23 lymphoblastoid cell lines (LCL) derived from BRCA1 mutation carriers and non-carriers. We have identified that this SNP is associated to a significant OGG1 transcriptional down regulation independently of the BRCA mutational status and that the variant may exert a synergistic effect together with BRCA1 or BRCA2 mutations on DNA damage and telomere shortening. These results suggest that this variant, could be associated to a higher cancer risk in BRCA1 mutation carriers, due to an OGG1 transcriptional down regulation and its effect on genome instability.