Portal de Periódicos da CAPES

Expression and purification of rhodopsin and its mutants from stable mammalian cell lines: application to NMR studies

1999; Portland Press; Volume: 27; Issue: 6 Linguagem: Inglês

10.1042/bst0270950

1470-8752

Philip J. Reeves, Judith Klein‐Seetharaman, E. V. Getmanova, Markus Eilers, Michèle C. Loewen, Steven O. Smith, H. Gobind Khorana,

Neuroscience and Neuropharmacology Research

Conference Article| December 01 1999 Expression and purification of rhodopsin and its mutants from stable mammalian cell lines: application to NMR studies Philip J. Reeves; Philip J. Reeves 1 *Departments of Biology and Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, U.S.A. 1To whom correspondence should be addressed. Search for other works by this author on: This Site PubMed Google Scholar Judith Klein-Seetharaman; Judith Klein-Seetharaman *Departments of Biology and Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Elena V. Getmanova; Elena V. Getmanova *Departments of Biology and Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Markus Eilers; Markus Eilers †Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794–5215, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Michèle C. Loewen; Michèle C. Loewen *Departments of Biology and Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar Steven O. Smith; Steven O. Smith †Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY 11794–5215, U.S.A. Search for other works by this author on: This Site PubMed Google Scholar H. Gobind Khorana H. Gobind Khorana 1 *Departments of Biology and Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, U.S.A. 1To whom correspondence should be addressed. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1999) 27 (6): 950–955. https://doi.org/10.1042/bst0270950 Article history Received: June 21 1999 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation Philip J. Reeves, Judith Klein-Seetharaman, Elena V. Getmanova, Markus Eilers, Michèle C. Loewen, Steven O. Smith, H. Gobind Khorana; Expression and purification of rhodopsin and its mutants from stable mammalian cell lines: application to NMR studies. Biochem Soc Trans 1 December 1999; 27 (6): 950–955. doi: https://doi.org/10.1042/bst0270950 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: WT, wild-type, ROS, rod outer segment(s), DM, dodecyl maltoside, FBS, fetal bovine serum, DMEM, Dulbecco's modified Eagle's medium, mAb, monoclonal antibody, 293S, suspension-adapted human embryonic kidney 293 This content is only available as a PDF. © 1999 Biochemical Society1999 Article PDF first page preview Close Modal You do not currently have access to this content.