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Analysis of DDK Syndrome Related Gene by Real-time PCR

2013; Henan Academy of Agriculture Sciences; Linguagem: Inglês

1004-3268

Zheng Zhen-yu,

RNA Research and Splicing

In order to screen the genes associated with DDK syndrome,four significantly differential expression genes including EGF,Bcl-2,Mcl-1,and EGF-R were analyzed by RT-PCR.The8-10 weeks old mice of clean grade were mated in accordance in the way that male and female ratio was 1∶1,using the DDK♀×BALB/c♂ mating combination as the experimental group while the BALB/c♀×BALB/c♂ mating combination as control group.According to embryonic development,embryos implanted in the early 2-cell,4-cell,and 8-cell stages were observed and collected.The total RNAs were extracted,reversly transcribed and analyzed using quantitative PCR technique to compare the expression differences of selected genes in different mating combinations and different stages of embryos.The expression differences of three genes,EGF,Bcl-2,and Mcl-1,in the three periods were extremely significant while the expression difference of EGF-R was extremely significant in the 4-cell and 8-cell stages,suggesting that the four candidate genes were related to the DDK syndrome.