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Effects of achyranthes bidentata polysaccharides on eosinophil and mast cells in airway of young as thmatic rats

2006; Chinese Pharmacological Society; Linguagem: Inglês

1001-1978

Aimin Huang,

Seaweed-derived Bioactive Compounds

Aim To investigate changes of eosinophil(EOS) and mast cells(MC) in airway of young asthmatic rats and to evaluate of achyranthes bidentata polysaccharides(ABPS) on it.Methods Fifty Sprague-Dawley(SD) rats were randomly divided into five groups,ten rats per group:asthmatic group(A),control group(C),pretreatment groups with ABPS which was done according to three different schedules:consecutively 3 days at sensitization(T_1),at challenge(T_2) or both of the two periods(T_3).Asthmatic rats were induced by intraperitioneal sensitization and challenged with nebulized ovalbumin(OVA).Lungs were embeded in paraffine and sliced,Hematoxylin and eosin(HE) staind tobuidine blue restained and TUNEL method after animals were executed,then the quantity and degranulation phenomenon of EOS and MC and the apoptosis of EOS in airway were observed.Results ① The number of inflammatory cells and EOS in the airway was significantly increased in asthmatic group but reduced in group T_1 and T_3(P0.05,P0.01).There was significantly difference between A and T_1 and T_3 group.Though it was also decreased in group T_2,but there was no significance difference when compared with group A.② In group A,there were a few apoptotic EOS.The percentage of apoptotic EOS was distinctly lower in group A than that in group C[(5.3±2.2)% 、(15.9±2.4)%,(P0.01)].Compared with that in group A,it in group T_1 and T_3 was evidently increased [(8.7±2.9)%,(9.8±2.2)%,(P0.05)].③ The MC quantity and rate of degranulation in group T_1 and T_3 were evidently reduced.Compared with that in group A,the ratio of degranulation of MC in group T_1 and T_3 was evidently increased [(32.2±8.3)%,(28.4±6.6)%],and there was significantly difference(P0.05).Therestrain ratio in ABPS treated(T_1,T_2,T_3) was 26.7%,11.8%,35.3%.Conclusions In asthmatic rats,the number of inflammatory cell and EOS and MC was evidently increased.ABPS could not only reduce the number of EOS and MC in the lung but also enhance the apoptosis of EOS and inhibit the release of granune of MC.