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Comparison of in vitro Cytotoxicity of Silver Particles with Different Sizes

2007; Linguagem: Inglês

Ling Xiong,

Nanoparticles: synthesis and applications

Object To compare the cellular toxic response with respect to the different sizes of silver particles and investigate the relationship between size and toxicity.Methods Size distribution of silver particles was confirmed by scanning electron microscopy(SEM),L929 cells were used in this study.Silver particles with different sizes in various concentrations were contact with the cells respectively.The cellular morphology was observed by phase contrast inverted microscopy and proliferation of the cells was examined using mitochondrial function(MTT) assay.Relative growth rate(RGR) was calculated and cytotoxicity grade was evaluated.Results Silver nanoparticles showed low toxicity when silver concentrations within 2.5～25μg/mL,the cell RGR decreased as the concentration increasing.However,when silver concentration reached 50μg/mL,it showed significant toxicity and the dose-effect response disappeared at the same time.Silver microparticles of larger size showed significant toxicity when concentration reached 250μg/mL.Among all concentrations,only cells contacted with silver microparticles of larger size showed normal morphology and proliferation.Conclusions There was different cytotoxicity between nanoscale and microscale silver.It showed silver nanoparticles and smaller silver microparticles have higher cytotoxicity than lager silver microparticles at the same concentrations.