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Ginsenoside Rg1 and platelet-rich fibrin enhance human breast adipose-derived stem cells function for soft tissue regeneration

2016; Impact Journals LLC; Volume: 7; Issue: 23 Linguagem: Inglês

10.18632/oncotarget.9360

1949-2553

Fang-Tian Xu, Zhi-Jie Liang, Hongmian Li, Qiliu Peng, Minhong Huang, De-Quan Li, Yi-Dan Liang, Gang‐Yi Chi, De-Hui Li, Bing-Chao Yu, Jirong Huang,

Wound Healing and Treatments

// Fang-Tian Xu 1, * , Zhi-Jie Liang 2, * , Hong-Mian Li 3 , Qi-Liu Peng 4 , Min-Hong Huang 2 , De-Quan Li 5 , Yi-Dan Liang 4 , Gang-Yi Chi 3 , De-Hui Li 6 , Bing-Chao Yu 6 , Ji-Rong Huang 6 1 Department of Orthopedics, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China 2 Department of Hepatobiliary and Gland Surgery, The Fifth Affiliated Hospital of Guangxi Medical University & The First People's Hospital of Nanning, Nanning 530022, China 3 Department of Plastic and Aesthetic Surgery, The Fifth Affiliated Hospital of Guangxi Medical University & The First People's Hospital of Nanning, Nanning 530022, China 4 Central Laboratory of Medical Science, The Fifth Affiliated Hospital of Guangxi Medical University & The First People's Hospital of Nanning, Nanning 530022, China 5 Department of Breast Surgery, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China 6 Department of Burns and Plastic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China * These authors have contributed equally to this work as co-first authors Correspondence to: Hong-Mian Li, e-mail: doctorlee2228@163.com Keywords: human breast adipose-derived stem cells, collagen type I sponge scaffolds, ginsenoside Rg1, platelet rich fibrin, soft tissue regeneration Received: November 20, 2015 Accepted: April 11, 2016 Published: May 14, 2016 ABSTRACT Adipose-derived stem cells (ASCs) can be used to repair soft tissue defects, wounds, burns, and scars and to regenerate various damaged tissues. The cell differentiation capacity of ASCs is crucial for engineered adipose tissue regeneration in reconstructive and plastic surgery. We previously reported that ginsenoside Rg1 (G-Rg1 or Rg1) promotes proliferation and differentiation of ASCs in vitro and in vivio . Here we show that both G-Rg1 and platelet-rich fibrin (PRF) improve the proliferation, differentiation, and soft tissue regeneration capacity of human breast adipose-derived stem cells (HBASCs) on collagen type I sponge scaffolds in vitro and in vivo . Three months after transplantation, tissue wet weight, adipocyte number, intracellular lipid, microvessel density, and gene and protein expression of VEGF, HIF-1α, and PPARγ were higher in both G-Rg1- and PRF-treated HBASCs than in control grafts. More extensive new adipose tissue formation was evident after treatment with G-Rg1 or PRF. In summary, G-Rg1 and/or PRF co-administration improves the function of HBASCs for soft tissue regeneration engineering.