Portal de Periódicos da CAPES

Use of Elevated Reverse Transcription Reaction Temperatures in RT-PCR

1996; Future Science Ltd; Volume: 20; Issue: 5 Linguagem: Inglês

10.2144/96205bm09

1940-9818

Willard M. Freeman, Sheila L. Vrana, Kent E. Vrana,

RNA Research and Splicing

BioTechniquesVol. 20, No. 5 BenchmarksOpen AccessUse of Elevated Reverse Transcription Reaction Temperatures in RT-PCRWillard M. Freeman, Sheila L. Vrana & Kent E. VranaWillard M. FreemanThe Bowman Gray School, of Medicine, Winston-Salem, NC, USA, Sheila L. VranaThe Bowman Gray School, of Medicine, Winston-Salem, NC, USA & Kent E. Vrana*Address correspondence to Kent E. Vrana, Department of Physiology and Pharmacology, Bowman Gray School of Medicine, Medical Center Blvd., Winston-Salem, NC 27157-1083, USA. Internet: E-mail Address: kvrana@medcenter.wpmail.wfu.eduThe Bowman Gray School, of Medicine, Winston-Salem, NC, USAPublished Online:2 Aug 2018https://doi.org/10.2144/96205bm09AboutSectionsPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinkedInRedditEmail "Use of Elevated Reverse Transcription Reaction Temperatures in RT-PCR." , 20(5), pp. 782–783FiguresReferencesRelatedDetailsCited ByBioactive Glasses in Gene Regulation and ProliferationMarker Development for Important Grapevine Traits by Genetic Diversity Studies and Investigation of Differential Gene Expression2 August 2010The road from qualitative to quantitative assay: What is next?Exploring the Correlation between Halorespirer Biomarker Concentrations and TCE Dechlorination RatesJournal of Environmental Engineering, Vol. 134, No. 11Structure-Function Analyses of Single Cells by Combining Patch-Clamp Techniques with Reverse Transcription-Polymerase Chain ReactionAn Internal Reference Technique for Accurately Quantifying Specific mRNAs by Real-Time PCR with Application to the tceA Reductive Dehalogenase GeneApplied and Environmental Microbiology, Vol. 71, No. 7Real Time Reverse Transcription PCR4 August 2011Detection of prolactin receptor mRNA in the corpus striatum and substantia nigra of the rat25 January 2002 | Journal of Neuroscience Research, Vol. 67, No. 4Structure and Function Analysis of the Calcium-Related Gene spray in Neurospora crassaFungal Genetics and Biology, Vol. 32, No. 3Fast Detection of Tissue Factor and Tissue Factor Pathway Inhibitor Messenger RNA in Endothelial Cells and Monocytes by Sensitive Reverse Transcription–Polymerase Chain ReactionThrombosis Research, Vol. 94, No. 3Quantitative RT-PCR: Pitfalls and PotentialWillard M. Freeman, Stephen J. Walker & Kent E. Vrana22 August 2018 | BioTechniques, Vol. 26, No. 1Developmental and Hormonal Regulation of Transforming Growth Factor-α and Epidermal Growth Factor Receptor Gene Expression in Isolated Prostatic Epithelial and Stromal Cells*Endocrinology, Vol. 139, No. 3 Vol. 20, No. 5 Follow us on social media for the latest updates Metrics Downloaded 281 times History Published online 2 August 2018 Published in print May 1996 Information© 2018 Author(s)PDF download