N-(2-Hydroxypropyl) Methacrylamide Based Cryogels – Synthesis and Biomimetic Modification for Stem Cell Applications
2015; Institute of Physiology of the Czech Academy of Sciences; Linguagem: Inglês
10.33549/physiolres.933134
ISSN1802-9973
AutoresAnna Golunova, Josef Jaroš, Veronika Jurtíková, Ilya Kotelnikov, J. Kotek, Helena Hlídková, Libor Streit, Aleš Hampl, František Rypáček, Vladimír Proks,
Tópico(s)Graphene and Nanomaterials Applications
ResumoThe design of favorable mechanical properties and suitable surface modifications of hydrogels in order to stimulate specific cell response is a great challenge. N-(2-Hydroxypropyl) methacryl-amide (HPMA) was utilized to form macroporous cryogel scaffolds for stem cell applications. Furthermore, one group of scaffolds was enhanced by copolymerization of HPMA with methacryloyl-GGGRGDS-OH peptide in an effort to integrate biomimetic adhesion sites. The cryogels were characterized by stiffness and equilibrium swelling measurements as well as by scanning electron microscopy. Cell culture experiments were performed with human adipose-derived stem cells and substrates were found completely non-toxic. Moreover, RGDS-enriched cryogels supported cell attachment, spreading and proliferation, so they can be considered suitable for designed aims.
Referência(s)