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Experimental Intravesical Therapy for Superficial Transitional Cell Carcinoma in a Rat Bladder Tumor Model

1991; Lippincott Williams & Wilkins; Volume: 145; Issue: 3 Linguagem: Inglês

10.1016/s0022-5347(17)38413-6

1527-3792

Gary D. Steinberg, Charles B. Brendler, Robert A. Squire, John T. Isaacs,

Urological Disorders and Treatments

No AccessJournal of Urology1 Mar 1991Experimental Intravesical Therapy for Superficial Transitional Cell Carcinoma in a Rat Bladder Tumor Model Gary D. Steinberg, Charles B. Brendler, Robert A. Squire, and John T. Isaacs Gary D. SteinbergGary D. Steinberg More articles by this author , Charles B. BrendlerCharles B. Brendler More articles by this author , Robert A. SquireRobert A. Squire More articles by this author , and John T. IsaacsJohn T. Isaacs More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(17)38413-6AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail A rodent bladder cancer model that is induced by intravesical instillation of N-methyl-N-nitrosourea (MNU) was characterized. Cohorts of four to five week old female Fisher 344 rats received four biweekly 1.5mg. doses of intravesical MNU and were sacrificed at various intervals. By week 13 all animals had flat atypia and/or papillary transitional cell tumors, and 67% of the lesions were moderately (grade II) or poorly differentiated (grade III). By week 20, 83% had gross muscle invasive tumors that eventually killed the host. A cohort of 40 MNU treated animals was subsequently treated commencing at week 17 after initiation of MNU with one of three intravesical six week regimens: 1) saline; 2) BCG (Tice strain); or 3) recombinant human tumor necrosis factor (RTNF) plus adriamycin. There was no difference in animal survival or tumor growth in any group of animals commencing therapy at week 17. A second cohort of 107 animals commenced therapy at 13 weeks after initiation of MNU with one of five intravesical six week regimens: 1) intravesical BCG (Tice strain); 2) adriamycin; 3) recombinant human tumor necrosis factor (RTNF); 4) RTNF plus adriamycin; or 5) BCG plus adriamycin. BCG, RTNF or adriamycin alone had no effect on tumor growth; however, BCG plus adriamycin and RTNF plus adriamycin commencing at week 13 significantly inhibited tumor growth and progression. In conclusion, this autochthonous intravesical rodent transitional cell carcinoma model appears useful for the following reasons: 1) it closely resembles human transitional cell carcinoma histologically and biologically in that all animals develop neoplastic changes in-situ that progress to muscle invasion and kill the host; 2) as with human bladder cancer these tumors do not respond to intravesical therapy if treated when tumor burden is large; however, tumor growth is inhibited when treated early; and 3) this model appears appropriate for screening and developing new intravesical treatments for superficial bladder cancer. © 1991 by The American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited ByWANG Z, DURAND D, SCHOENBERG M and PAN Y (2018) FLUORESCENCE GUIDED OPTICAL COHERENCE TOMOGRAPHY FOR THE DIAGNOSIS OF EARLY BLADDER CANCER IN A RAT MODELJournal of Urology, VOL. 174, NO. 6, (2376-2381), Online publication date: 1-Dec-2005.Okamura T, Tozawa K, Yamada Y, Sakagami H, Ueda K and Kohri K (2018) Clinicopathological Evaluation of Repeated Courses of Intravesical Bacillus Calmette-Guerin Instillation for Preventing Recurrence of Initially Resistant Superficial Bladder CancerJournal of Urology, VOL. 156, NO. 3, (967-971), Online publication date: 1-Sep-1996. Volume 145Issue 3March 1991Page: 647-653 Advertisement Copyright & Permissions© 1991 by The American Urological Association Education and Research, Inc.Keywordsbladder cancerrecombinant human tumor necrosis factoradriamycinbacillus Calmette-GuerinMetricsAuthor Information Gary D. Steinberg More articles by this author Charles B. Brendler More articles by this author Robert A. Squire More articles by this author John T. Isaacs More articles by this author Expand All Advertisement Loading ...